Dna hypomethylation of individual sequences in aborted cloned bovine fetuses
文献类型：期刊论文
| 作者 | Chen, T; Jiang, Y; Zhang, YL; Liu, JH; Hou, Y; Schatten, H; Chen, DY; Sun, QY |
| 刊名 | Frontiers in bioscience
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 10页码:3002-3008 |
| 关键词 | Reproduction Dna methylation Nuclear transfer Bovine Cow Fetus Abortion |
| ISSN号 | 1093-9946 |
| 通讯作者 | Sun, qy() |
| 英文摘要 | Cloned bovines have a much higher abortion rate than those derived in vivo. available evidence indicates that inappropriate epigenetic reprogramming of donor nuclei is the primary cause of cloning failure. to gain a better understanding of the dna methylation changes associated with the high abortion rate of cloned bovines, we examined the dna methylation status of a repeated sequence (satellite i) and the promoter regions of two single-copy genes (interleukin 3/cytokeratin) in aborted cloned fetuses, aborted fetuses derived from artificial insemination (ai), cloned adults and ai adults by bisulfite sequencing and restriction enzyme analysis. two of four aborted cloned fetuses show very low methylation levels in the two single-copy gene promoter regions. one of the two fetuses also showed undermethylated status in the satellite i sequence. the other two aborted cloned fetuses have similar methylation levels to those of aborted ai fetuses. however, no difference in methylation was observed between cloned adults and ai adults. our results demonstrate for the first time the undermethylated status of individual sequences in aborted cloned fetuses. these findings suggest that aberrant dna methylation may contribute to the developmental failure of cloned bovine fetuses. |
| WOS关键词 | CHROMOSOME METHYLATION PATTERNS ; DE-NOVO METHYLATION ; NUCLEAR TRANSFER ; SOMATIC-CELL ; PREIMPLANTATION DEVELOPMENT ; MAMMALIAN DEVELOPMENT ; PATERNAL GENOME ; DONOR GENOME ; IN-VIVO ; EMBRYOS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000232320300083 |
| 出版者 | FRONTIERS IN BIOSCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377117 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, QY |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA |
| 推荐引用方式 GB/T 7714 | Chen, T,Jiang, Y,Zhang, YL,et al. Dna hypomethylation of individual sequences in aborted cloned bovine fetuses[J]. Frontiers in bioscience,2005,10:3002-3008. |
| APA | Chen, T.,Jiang, Y.,Zhang, YL.,Liu, JH.,Hou, Y.,...&Sun, QY.(2005).Dna hypomethylation of individual sequences in aborted cloned bovine fetuses.Frontiers in bioscience,10,3002-3008. |
| MLA | Chen, T,et al."Dna hypomethylation of individual sequences in aborted cloned bovine fetuses".Frontiers in bioscience 10(2005):3002-3008. |
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来源：中国科学院大学
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