Influence of membrane physical state on lysosomal potassium ion permeability
文献类型：期刊论文
| 作者 | Wang, J; Zhang, GJ |
| 刊名 | Cell biology international
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 29期号:6页码:393-401 |
| 关键词 | Lysosomes Membrane fluidity Potassium ion permeability Membrane potential |
| ISSN号 | 1065-6995 |
| DOI | 10.1016/j.cellbi.2004.12.002 |
| 通讯作者 | Zhang, gj() |
| 英文摘要 | Lysosomal permeability to potassium ions is an important property of the organelle. influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal proton leakage with p-nitrophenol. the membrane fluidity of lysosomes was modulated by treatment with membrane fluidizer benzyl alcohol and rigiditier cholesteryl hemisuccinate. changes in the membrane order were examined by steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene. the measurements of membrane potential and proton leakage demonstrated that the permeability of lysosomes to potassium ions increased with rigidification of their membranes by cholesteryl hemisuccinate treatment at 37 degrees c, and decreased with fluidization of their membranes by benzyl alcohol treatment at 2 degrees c. the changes in ion permeability could be recovered by fluidizing the rigidified membranes and rigidifying the fluidized membranes. the results suggest that the physical states of lysosomal membranes play an important role in the regulation of their k+ permeability. (c) 2005 international federation for cell biology. published by elsevier ltd. all rights reserved. |
| WOS关键词 | PROTON PUMP ; RAT-LIVER ; CATION PERMEABILITY ; LIPID-MEMBRANES ; CELL-MEMBRANES ; FLUIDITY ; TRANSPORT ; INCREASE ; PH ; DESTABILIZATION |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000231189200001 |
| 出版者 | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377164 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, GJ |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, J,Zhang, GJ. Influence of membrane physical state on lysosomal potassium ion permeability[J]. Cell biology international,2005,29(6):393-401. |
| APA | Wang, J,&Zhang, GJ.(2005).Influence of membrane physical state on lysosomal potassium ion permeability.Cell biology international,29(6),393-401. |
| MLA | Wang, J,et al."Influence of membrane physical state on lysosomal potassium ion permeability".Cell biology international 29.6(2005):393-401. |
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来源：中国科学院大学
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