Screening of inhibitors for influenza a virus using high-performance affinity chromatography and combinatorial peptide libraries
文献类型：期刊论文
| 作者 | Zhao, R; Fang, CL; Yu, X; Liu, Y; Luo, J; Shangguan, D; Xiong, SX; Su, TS; Liu, GQ |
| 刊名 | Journal of chromatography a
 |
| 出版日期 | 2005-01-28 |
| 卷号 | 1064期号:1页码:59-66 |
| ISSN号 | 0021-9673 |
| 关键词 | High-performance affinity chromatography Combinatorial peptide library Inhibitor Influenza a virus Screening |
| 通讯作者 | Zhao, r(zhaorui@iccas.ac.cn) |
| 英文摘要 | The affinity inhibitor of fusion peptide of influenza a virus has been studied using a combination of high-performance affinity, chromatography (hpac) and combinatorial peptide libraries. fusion peptide (fp) (1-11) of influenza a virus was used as the affinity ligand and immobilized onto the poly(glycidyl methacrylate) (pgma) beads. positional scanning peptide libraries based on antisense peptide strategy and extended peptide libraries were designed and synthesized. the screening was carried out at acidic ph (5.5) in order to imitate the environment of virus fusion. a hendecapeptide fhrkkgrgkhk was identified to have a strong affinity to the fp (1-11). the dissociation constant of the complex of the hendecapeptide and the fp (1-11) is 3.10 x 10(-6) mol l(-1) in a physiological buffer condition. the polypeptide has a fairly inhibitory effect on three different strains of influenza a virus hlnl subtype. (c) 2004 elsevier b.v. all rights reserved. |
| WOS关键词 | BINDING ; POLYMERIZATION ; HEMAGGLUTININ ; FUSION ; BEADS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000226706700007 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377190 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, R |
| 作者单位 | 1.Chinese Acad Sci, Ctr Mol Sci, Inst Chem, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, R,Fang, CL,Yu, X,et al. Screening of inhibitors for influenza a virus using high-performance affinity chromatography and combinatorial peptide libraries[J]. Journal of chromatography a,2005,1064(1):59-66. |
| APA | Zhao, R.,Fang, CL.,Yu, X.,Liu, Y.,Luo, J.,...&Liu, GQ.(2005).Screening of inhibitors for influenza a virus using high-performance affinity chromatography and combinatorial peptide libraries.Journal of chromatography a,1064(1),59-66. |
| MLA | Zhao, R,et al."Screening of inhibitors for influenza a virus using high-performance affinity chromatography and combinatorial peptide libraries".Journal of chromatography a 1064.1(2005):59-66. |
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来源：中国科学院大学
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