A new method for purification of recombinant human alpha-synuclein in escherichia coli
文献类型：期刊论文
| 作者 | Huang, CJ; Ren, GP; Zhou, H; Wang, CC |
| 刊名 | Protein expression and purification
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 42期号:1页码:173-177 |
| 关键词 | Alpha-synuclein Purification Escherichia coli Periplasm Osmotic shock |
| ISSN号 | 1046-5928 |
| DOI | 10.1016/j.pep.2005.02.014 |
| 通讯作者 | Wang, cc() |
| 英文摘要 | Alpha-synuclein (as), a major component of lewy body in parkinson's disease patients, exists as a natively unfolded protein in physiological buffer. we recently found that the overexpressed as in escherichia coli bearing the cloned as cdna with no signal sequence was actually located inside the periplasm. but not in the cytoplasm as generally recognized. therefore, a new protocol for preparing recombinant as has been developed with only two steps: (1) osmotic shock for release of as-containing periplasm fraction and (2) ion-exchange chromatography for further purification of as. by using plasmids and e coli strains commonly used the new protocol is much more convenient, faster, and cheaper compared to the current methods established since 1994. about 80 mg as with 95% purity can be regularly prepared from a 1 l culture in 3 days. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | PARKINSONS-DISEASE ; PROTEINS ; AGGREGATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000229915500023 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377202 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, CC |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, CJ,Ren, GP,Zhou, H,et al. A new method for purification of recombinant human alpha-synuclein in escherichia coli[J]. Protein expression and purification,2005,42(1):173-177. |
| APA | Huang, CJ,Ren, GP,Zhou, H,&Wang, CC.(2005).A new method for purification of recombinant human alpha-synuclein in escherichia coli.Protein expression and purification,42(1),173-177. |
| MLA | Huang, CJ,et al."A new method for purification of recombinant human alpha-synuclein in escherichia coli".Protein expression and purification 42.1(2005):173-177. |
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来源：中国科学院大学
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