A human sars-cov neutralizing antibody against epitope on s2 protein
文献类型：期刊论文
| 作者 | Duan, JZ; Yan, XY; Guo, XM; Cao, WC; Han, W; Qi, C; Feng, J; Yang, DL; Gao, GX; Jin, G |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-07-22 |
| 卷号 | 333期号:1页码:186-193 |
| 关键词 | Sars-cov Neutralizing antibody S2 protein Scfv Phage-display library |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.05.089 |
| 通讯作者 | Yan, xy() |
| 英文摘要 | An immune antibody phage-display library was constructed from b cells of sars convalescent patients. more than 80 clones were selected from the library by using the whole inactivated sars-cov virions as target. one human scfv, b1, was characterized extensively. the b1 recognized sars pseudovirus in vivo and competed with sars sera for binding to sars-cov with high affinity (equilibrium dissociation constant, k-d = 105 nm). the b1 also has potent neutralizing activities against infection by pseudovirus expressing sars-cov s protein in vitro. finally, we found that the b1 recognized an epitope on s2 protein, especially within amino acids 1023-1189 of s2 protein. this study not only first made a human neutralizing antibody, which recognized an epitope on s2 protein like natural antibody in sera, but also may help us to better understand the immunological characteristics of sars protein and sars vaccine design. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | ACUTE-RESPIRATORY-SYNDROME ; CORONAVIRUS SPIKE GLYCOPROTEIN ; ANGIOTENSIN-CONVERTING ENZYME-2 ; POTENT NEUTRALIZATION ; CONVALESCENT PLASMA ; IDENTIFICATION ; RECEPTOR ; ELLIPSOMETRY ; LIBRARIES ; FRAGMENT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000230105200027 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377211 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yan, XY |
| 作者单位 | 1.Inst Biophys, Natl Lab Biomacromol, Beijing, Peoples R China 2.Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Inst Microbiol & Epidemiol, Dept Epidemiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, JZ,Yan, XY,Guo, XM,et al. A human sars-cov neutralizing antibody against epitope on s2 protein[J]. Biochemical and biophysical research communications,2005,333(1):186-193. |
| APA | Duan, JZ.,Yan, XY.,Guo, XM.,Cao, WC.,Han, W.,...&Jin, G.(2005).A human sars-cov neutralizing antibody against epitope on s2 protein.Biochemical and biophysical research communications,333(1),186-193. |
| MLA | Duan, JZ,et al."A human sars-cov neutralizing antibody against epitope on s2 protein".Biochemical and biophysical research communications 333.1(2005):186-193. |
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来源：中国科学院大学
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