Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3
文献类型：期刊论文
| 作者 | Zhang, D; Pan, L; Yang, LH; He, XK; Huang, XY; Sun, FZ |
| 刊名 | Human reproduction
 |
| 出版日期 | 2005-11-01 |
| 卷号 | 20期号:11页码:3053-3061 |
| 关键词 | Calcium oscillations Embryos Insp3r Oocytes Sr2+ |
| ISSN号 | 0268-1161 |
| DOI | 10.1093/humrep/dei215 |
| 通讯作者 | Sun, fz() |
| 英文摘要 | Background: sr2+ is the most efficient agent for mouse oocyte activation and functions by inducing ca2+ oscillations. however, its specific mechanism of action remains unknown. here we investigated the specificity and possible mechanism of sr2+-induced ca2+ oscillations in mouse oocytes and early embryos. methods: ca2+ oscillations in oocytes and embryos were measured by ratiometric fluorescence imaging using fura-2am. the role of phospholipase c (plc) and inositol trisphosphate (insp3) receptors in sr2+-induced ca2+ oscillations was examined by selective inhibitors. results: sr2+ can induce ca2+ oscillations in both immature and mature oocytes, and in early embryos. a cell cycle stage-dependent phenomenon to sr2+ stimulation was observed in 1-cell embryos. by using a low molecular weight heparin to antagonize the function of insp3 receptors, we were able to show that insp3 receptors are essential for sr2+-induced ca2+ oscillations. treating metaphase ii (mii) oocytes with the plc inhibitor, u73122, abolished sr2+-induced increases in ca2+. this inhibitory effect of u73122 could be rescued by microinjection of insp3, indicating that sr2+-induced ca2+ oscillations require the synergistic action of insp3. conclusions: sr2+-induced calcium oscillations in mouse oocytes and early embryos are mediated through insp3 receptors, and require plc activation and the synergistic action of insp3. |
| WOS研究方向 | Obstetrics & Gynecology ; Reproductive Biology |
| WOS类目 | Obstetrics & Gynecology ; Reproductive Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000233045700013 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377244 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, FZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, D,Pan, L,Yang, LH,et al. Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3[J]. Human reproduction,2005,20(11):3053-3061. |
| APA | Zhang, D,Pan, L,Yang, LH,He, XK,Huang, XY,&Sun, FZ.(2005).Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3.Human reproduction,20(11),3053-3061. |
| MLA | Zhang, D,et al."Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3".Human reproduction 20.11(2005):3053-3061. |
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来源：中国科学院大学
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