Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from achromobacter xylosoxidans
文献类型：期刊论文
| 作者 | Cai, G; Zhu, SC; Wang, XJ; Jiang, WH |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 249期号:1页码:15-21 |
| 关键词 | Achromobacter xylosoxidans Amidase Amidase signature family Ana Gc content Wide-spectrum |
| ISSN号 | 0378-1097 |
| DOI | 10.1016/j.femsle.2005.05.038 |
| 通讯作者 | Jiang, wh() |
| 英文摘要 | Amidases are very important enzymes for industrial biocatalysis. we scored a novel amidase by screening the achromobacter xylosoxidans gene library with cephalosporin analogous amides. the gene coding for the enzyme, designated ana, was cloned, sequenced and overexpressed in escherichia coli. sequence analysis of ana showed it to be an amidase signature family member. interestingly, we noted that almost all ana homologous amidases are from human pathogens responsible for chronic lung infections. knowing, the genetic context of ana and its homologous amidases, we suggest that they could be a part of transposon structure. ana can efficiently hydrolyze a series of cephalosporin analogous amides, including amides with an aninine, p-nitro-aninine, and beta-naphthylamine moiety, while cephalosporin could not serve as its substrate. (c) 2005 published by elsevier b.v. on behalf of the federation of european microbiological societies. |
| WOS关键词 | ESCHERICHIA-COLI ; ACYLASE ACTIVITY ; STRAIN |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000230939300003 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377281 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, WH |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Mol Microbiol Lab, Shanghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Fudan Univ, Sch Publ Hlth, Dept Prevent Med, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, G,Zhu, SC,Wang, XJ,et al. Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from achromobacter xylosoxidans[J]. Fems microbiology letters,2005,249(1):15-21. |
| APA | Cai, G,Zhu, SC,Wang, XJ,&Jiang, WH.(2005).Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from achromobacter xylosoxidans.Fems microbiology letters,249(1),15-21. |
| MLA | Cai, G,et al."Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from achromobacter xylosoxidans".Fems microbiology letters 249.1(2005):15-21. |
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来源：中国科学院大学
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