Crystal structure of earthworm fibrinolytic enzyme component b: a novel, glycosylated two-chained trypsin
文献类型：期刊论文
| 作者 | Wang, F; Wang, C; Li, M; Zhang, JP; Gui, LL; An, XM; Chang, WR |
| 刊名 | Journal of molecular biology
 |
| 出版日期 | 2005-05-06 |
| 卷号 | 348期号:3页码:671-685 |
| 关键词 | Earthworm fibrinolytic enzyme Crystal structure N-glycosylation Eight-membered ring Stability |
| ISSN号 | 0022-2836 |
| DOI | 10.1016/j.jmb.2005.02.055 |
| 通讯作者 | Chang, wr() |
| 英文摘要 | The earthworm fibrinolytic enzyme (efe), belonging to a group of serine proteases with strong fibrinolytic activity, has been used in a mixture as an oral drug for prevention and treatment of thrombosis in east asia. the efe component b (efe-b) is one of seven efe components from eisenia fetida, and among them it has nearly the highest fibrinolytic activity. here, we report its crystal structure at a resolution of 2.06 angstrom. the structural analysis shows that efe-b should be classified as a trypsin from earthworm. however, it is distinct from other trypsins. it is a two-chained protease with an n-terminal, pyroglutamated light chain and an n-glycosylated heavy chain. furthermore, the heavy chain contains a novel structural motif, an eight-membered ring resulting from a disulfide bridge between two neighboring cysteine residues, and a cis peptide bond exists between these two cysteine residues. the crystal structure of efe-b provides the structural basis for its high level of stability and reveals its complicated post-translational modifications in earthworm. this structure is the first reported for a glycosylated two-chained trypsin, which may provide useful clues to explain the origin and evolution of the chymotrypsin family. (c) 2005 elsevier ltd. all rights reserved. |
| WOS关键词 | PROTEIN SECONDARY STRUCTURE ; LUMBRICUS-RUBELLUS ; EISENIA-FOETIDA ; MOLECULAR-MECHANISMS ; SERINE PROTEASES ; CRYSTALLIZATION ; DETERMINANTS ; PROGRAM ; MODELS ; THERMOSTABILITY |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000228711100015 |
| 出版者 | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377293 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chang, WR |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, F,Wang, C,Li, M,et al. Crystal structure of earthworm fibrinolytic enzyme component b: a novel, glycosylated two-chained trypsin[J]. Journal of molecular biology,2005,348(3):671-685. |
| APA | Wang, F.,Wang, C.,Li, M.,Zhang, JP.,Gui, LL.,...&Chang, WR.(2005).Crystal structure of earthworm fibrinolytic enzyme component b: a novel, glycosylated two-chained trypsin.Journal of molecular biology,348(3),671-685. |
| MLA | Wang, F,et al."Crystal structure of earthworm fibrinolytic enzyme component b: a novel, glycosylated two-chained trypsin".Journal of molecular biology 348.3(2005):671-685. |
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来源：中国科学院大学
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