Subcellular localization of the s locus f-box protein ahslf-s-2 in pollen and pollen t tubes of self-incompatible antirrhinum
文献类型:期刊论文
作者 | Wang, HY; Xue, YB |
刊名 | Journal of integrative plant biology
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出版日期 | 2005 |
卷号 | 47期号:1页码:76-83 |
关键词 | Antirrhinum Pollen tube Self-incompatibility S locus f-box protein Subcellular localization |
ISSN号 | 1672-9072 |
通讯作者 | Xue, yb() |
英文摘要 | The distribution of the s locus f-box (slf) protein was examined by immunocytochemistry and western blot techniques using an antibody against the c-terminal part of ahslf-s-2 in self-incompatible lines of antirrhinum. abundant gold particles were found where pollen tubes emerge in vitro. with the elongation of pollen tubes, binding sites for the antibody were found in the cytoplasm of the pollen tubes, including the peripheral part of the endoplasmic reticulum. after germination in vitro for 16 h, the product of ahslf-s-2 and possibly its allelic products could still be detectable, implying that the slf protein has a role in the elongating process of pollen tubes. the present study provides evidence at the protein level that the slf protein is present in pollen cytoplasm during pollen tube growth. these findings are discussed, as is their potential role in the self-incompatible response in antirrhinum. |
WOS关键词 | NICOTIANA-ALATA ; TRANSCRIPTIONAL ANALYSIS ; PROTEASOME PATHWAY ; ALLELIC DIVERSITY ; PETUNIA-INFLATA ; GENE ; UBIQUITIN ; REJECTION ; RNASE ; IDENTIFICATION |
WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
语种 | 英语 |
WOS记录号 | WOS:000227090100009 |
出版者 | BLACKWELL PUBLISHING ASIA |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377309 |
专题 | 中国科学院大学 |
通讯作者 | Xue, YB |
作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, HY,Xue, YB. Subcellular localization of the s locus f-box protein ahslf-s-2 in pollen and pollen t tubes of self-incompatible antirrhinum[J]. Journal of integrative plant biology,2005,47(1):76-83. |
APA | Wang, HY,&Xue, YB.(2005).Subcellular localization of the s locus f-box protein ahslf-s-2 in pollen and pollen t tubes of self-incompatible antirrhinum.Journal of integrative plant biology,47(1),76-83. |
MLA | Wang, HY,et al."Subcellular localization of the s locus f-box protein ahslf-s-2 in pollen and pollen t tubes of self-incompatible antirrhinum".Journal of integrative plant biology 47.1(2005):76-83. |
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来源:中国科学院大学
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