Qcm-fia with pgma coating for dynamic interaction study of heparin and antithrombin iii
文献类型：期刊论文
| 作者 | Zhang, HW; Zhao, R; Chen, ZY; Shangguan, DH; Liu, GQ |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2005-07-15 |
| 卷号 | 21期号:1页码:121-127 |
| 关键词 | Qcm-fia Pgma coating Dynamic interaction Heparin At iii |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2004.08.041 |
| 通讯作者 | Zhao, r() |
| 英文摘要 | In this work, we describe a method of constructing a film of linear poly(glycidyl methacrylate) (pgma) polymer onto the surface of quartz crystal microbalance (qcm) electrode as a coating material that allows easy coupling of heparin molecules onto the electrode and facilitates the determination of the interaction between heparin and antithrombin iii (at iii). the pgma film was characterized with atomic force microscopy (afm) and infra-red spectroscopy. the coupling of heparin was accomplished in one step solution reaction. a home-made quartz crystal microbalance-flow injection analysis (qcm-fia) system with data analysis software developed in our laboratory was used to determine the interaction. the interactions between immobilized heparin and at ill were studied with various concentrations under various conditions. the obtained constants are k(ass) = (1.49 +/- 0.12) x 10(3) mol(-1) 1 s(-1), k(diss) = (3.94 +/- 0.63) x 10(-2) s(-1), k-a = (3.82 +/- 0.33) x 10(4) mol(-1)l. (c) 2004 elsevier b.v. all rights reserved. |
| WOS关键词 | QUARTZ-CRYSTAL MICROBALANCE ; PIEZOELECTRIC BIOSENSOR ; IN-SITU ; IMMOBILIZATION METHODS ; PROTEIN ADSORPTION ; IMMUNOSENSOR ; BINDING ; SENSOR ; SPECTROSCOPY ; SALMONELLA |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000232268900014 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377315 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, R |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, HW,Zhao, R,Chen, ZY,et al. Qcm-fia with pgma coating for dynamic interaction study of heparin and antithrombin iii[J]. Biosensors & bioelectronics,2005,21(1):121-127. |
| APA | Zhang, HW,Zhao, R,Chen, ZY,Shangguan, DH,&Liu, GQ.(2005).Qcm-fia with pgma coating for dynamic interaction study of heparin and antithrombin iii.Biosensors & bioelectronics,21(1),121-127. |
| MLA | Zhang, HW,et al."Qcm-fia with pgma coating for dynamic interaction study of heparin and antithrombin iii".Biosensors & bioelectronics 21.1(2005):121-127. |
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来源：中国科学院大学
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