Crucial role of the sarcoplasmic reticulum in the developmental regulation of ca2+ transients and contraction in cardiomyocytes derived from embryonic stem cells
文献类型：期刊论文
| 作者 | Fu, JD; Li, J; Tweedie, D; Yu, HM; Chen, L; Wang, R; Riordon, DR; Brugh, SA; Wang, SQ; Boheler, KR |
| 刊名 | Faseb journal
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 19期号:12页码:181-+ |
| 关键词 | Cardiomyocyte differentiation Calcium transients Type 2 ryanodine receptor |
| ISSN号 | 0892-6638 |
| DOI | 10.1096/fj.05-4501fje |
| 通讯作者 | Yang, ht(htyang@sibs.ac.cn) |
| 英文摘要 | In adult myocardium, excitation-contraction coupling is critically regulated by sarcoplasmic reticulum (sr) ca2+ release via type 2 ryanodine receptor (ryr2), but generally, it is believed that sr-function is rudimentary in the fetal heart and in embryonic stem (es) cell-derived cardiomyocytes (escms), a possible source for cell replacement therapies. this study used wild-type (ryr2(+/+)) and ryr2 null ( ryr2(-/-)) escms as an in vitro model of cardiomyogenesis, together with pharmacological approaches and expression profiles of genes relevant for sr function, to elucidate the functional importance of ryr2 and sr on the regulation of ca2+ transients and contraction during early cardiomyocyte development. during differentiation of ryr2(+/+) escms, sr function developed progressively with increased basal cytosolic free ca2+ concentration ([ ca2+] (i)), enhanced frequency and amplitude, and decreased duration of ca2+ transients that were inhibited by ryanodine and thapsigargin. these functional traits correlated with sr ca2+ load and the expression of ryr2, serca2a, and phospholamban. ryr2-/- escms, comparatively, demonstrated a significantly prolonged time-to-peak and reduced frequency of ca2+ transients and contractions. beta-adrenergic stimulation of ryr2(+/+) escms increased the frequency and amplitude of ca2+ transients with differentiation but was much weaker in ryr2(-/-)escms. we conclude that functional sr and control of ryr2-mediated sr ca2+ release directly contribute to the spontaneous and beta-adrenergic receptor-stimulated contraction of escms, even at very immature stages of development. |
| WOS关键词 | BETA-ADRENERGIC STIMULATION ; CARDIAC MYOCYTES ; RYANODINE RECEPTOR ; INTRACELLULAR CALCIUM ; CHAMBER MYOCARDIUM ; MOUSE HEART ; RAT ; RELEASE ; DIFFERENTIATION ; CHANNELS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000232991100007 |
| 出版者 | FEDERATION AMER SOC EXP BIOL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377318 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, HT |
| 作者单位 | 1.Chinese Acad Sci, Mol Cardiol Lab, Inst Hlth Sci, SIBS, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China 3.Chinese Acad Sci, Key Lab Stem Cell Biol, SIBS, Shanghai 200025, Peoples R China 4.Chinese Acad Sci, Grad Sch, Shanghai 200025, Peoples R China 5.NIA, Cardiovasc Sci Lab, Baltimore, MD 21224 USA 6.Peking Univ, Coll Life Sci, Natl Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, JD,Li, J,Tweedie, D,et al. Crucial role of the sarcoplasmic reticulum in the developmental regulation of ca2+ transients and contraction in cardiomyocytes derived from embryonic stem cells[J]. Faseb journal,2005,19(12):181-+. |
| APA | Fu, JD.,Li, J.,Tweedie, D.,Yu, HM.,Chen, L.,...&Yang, HT.(2005).Crucial role of the sarcoplasmic reticulum in the developmental regulation of ca2+ transients and contraction in cardiomyocytes derived from embryonic stem cells.Faseb journal,19(12),181-+. |
| MLA | Fu, JD,et al."Crucial role of the sarcoplasmic reticulum in the developmental regulation of ca2+ transients and contraction in cardiomyocytes derived from embryonic stem cells".Faseb journal 19.12(2005):181-+. |
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来源：中国科学院大学
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