The antibodies directed against n-terminal heptad-repeat peptide of hrsv fusion protein and its analog-5-helix inhibit virus infection in vitro
文献类型：期刊论文
| 作者 | Ni, L; Zhao, LQ; Gao, GF; Qian, Y; Tien, P |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-06-17 |
| 卷号 | 331期号:4页码:1358-1364 |
| 关键词 | Hrsv Fusion protein Heptad-repeat region 5-helix Neutralizing antibody |
| ISSN号 | 0006-291X |
| 通讯作者 | Qian, y() |
| 英文摘要 | Human respiratory syncytial virus (hrsv) membrane fusion is promoted by the formation of a trimer-of-hairpins structure that brings the amino- and carboxyl-terminal regions of fusion (f) protein into close proximity. two heptad-repeat (hr1 and hr2) regions in f protein play an important role in this process. our previous study demonstrated that peptides derived from hr1 and hr2 regions of f protein were potent inhibitors of hrsv entry. here we showed that hr1 peptide and its analog denoted 5-helix which contained a central coiled-coil formed by three hr1s could induce highly potent antibody response in the immunized rabbits. both antibodies could recognize f1 domain of the f protein and inhibited hrsv entry with the neutralizing antibody titers of 1:61 and 1:115, respectively. these suggested that 5-helix could induce potent neutralizing antibody response and the central coiled-coil might be a highly conserved neutralization site for hrsv f protein. (c) 2005 published by elsevier inc. |
| WOS关键词 | RESPIRATORY SYNCYTIAL VIRUS ; VIRAL MEMBRANE-FUSION ; INFLUENZA-A VIRUS ; COILED-COIL ; POTENT INHIBITORS ; VACCINE ; IDENTIFICATION ; GLYCOPROTEIN ; ACTIVATION ; RESISTANCE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000229234500065 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377353 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qian, Y |
| 作者单位 | 1.Capital Inst Pediat, Beijing Municipal Lab Infect & Immun, Beijing 100020, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Dept Mol Virol, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Capital Inst Pediat, Beijing 100020, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ni, L,Zhao, LQ,Gao, GF,et al. The antibodies directed against n-terminal heptad-repeat peptide of hrsv fusion protein and its analog-5-helix inhibit virus infection in vitro[J]. Biochemical and biophysical research communications,2005,331(4):1358-1364. |
| APA | Ni, L,Zhao, LQ,Gao, GF,Qian, Y,&Tien, P.(2005).The antibodies directed against n-terminal heptad-repeat peptide of hrsv fusion protein and its analog-5-helix inhibit virus infection in vitro.Biochemical and biophysical research communications,331(4),1358-1364. |
| MLA | Ni, L,et al."The antibodies directed against n-terminal heptad-repeat peptide of hrsv fusion protein and its analog-5-helix inhibit virus infection in vitro".Biochemical and biophysical research communications 331.4(2005):1358-1364. |
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