Mammalian freeze-dried sperm can maintain their calcium oscillation-inducing ability when microinjected into mouse eggs
文献类型：期刊论文
| 作者 | Liu, QC; Chen, TE; Huang, XY; Sun, FZ |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-03-25 |
| 卷号 | 328期号:4页码:824-830 |
| 关键词 | Sperm Freeze-drying Calcium oscillations Oocyte Mouse |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.01.034 |
| 通讯作者 | Sun, fz() |
| 英文摘要 | Mammalian freeze-dried sperm can maintain their genetic integrity and event support full development to term when microinjected into mature oocytes. however, it is unknown whether freeze-dried sperm can still maintain their calcium oscillation-inducing capability. here, we microinjected mouse and bovine fieeze-dried sperm into mouse m i i oocytes and examined their calcium oscillation-inducing ability following intracytoplasmic sperm injection (icsi). two pieces of information are revealed. first, nearly all oocytes injected with a freeze-dried mouse sperm head or a bovine sperm showed fertilization-like calcium oscillations, indicating that freeze-drying treatment does not affect the activity of the sperm factor responsible for calcium oscillations. second, freeze-dried sperm exhibited high resistance to external temperature increase. this is shown by the finding that the freeze-dried sperm can maintain their calcium oscillation-inducing capacity even following exposure to 100 degreesc for 3 h. we therefore conclude that mammalian sperm can maintain their calcium oscillation-inducing capability following freeze-drying, rehydration, and icsi treatments. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | SOLUBLE-PROTEIN FACTOR ; CA2+ OSCILLATIONS ; SPERMATOZOA ; FERTILIZATION ; OOCYTES ; TRIGGER ; CYCLE ; ACTIVATION ; GENERATION ; TRANSIENTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000227233000003 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377451 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, FZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Lab Mol Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, QC,Chen, TE,Huang, XY,et al. Mammalian freeze-dried sperm can maintain their calcium oscillation-inducing ability when microinjected into mouse eggs[J]. Biochemical and biophysical research communications,2005,328(4):824-830. |
| APA | Liu, QC,Chen, TE,Huang, XY,&Sun, FZ.(2005).Mammalian freeze-dried sperm can maintain their calcium oscillation-inducing ability when microinjected into mouse eggs.Biochemical and biophysical research communications,328(4),824-830. |
| MLA | Liu, QC,et al."Mammalian freeze-dried sperm can maintain their calcium oscillation-inducing ability when microinjected into mouse eggs".Biochemical and biophysical research communications 328.4(2005):824-830. |
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来源：中国科学院大学
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