Effects of brefeldin a on pollen germination and tube growth. antagonistic effects on endocytosis and secretion
文献类型：期刊论文
| 作者 | Wang, QL; Kong, LG; Hao, HQ; Wang, XH; Lin, JX; Samaj, J; Baluska, F |
| 刊名 | Plant physiology
 |
| 出版日期 | 2005-12-01 |
| 卷号 | 139期号:4页码:1692-1703 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.105.069765 |
| 通讯作者 | Lin, jx() |
| 英文摘要 | We assessed the effects of brefeldin a (bfa) on pollen tube development in picea meyeri using fluorescent marker fm4-64 as a membrane-inserted endocytic/ recycling marker, together with ultrastructural studies and fourier transform infrared analysis of cell walls. bfa inhibited pollen germination and pollen tube growth, causing morphological changes in a dose-dependant manner, and pollen tube tip growth recovered after transferring into bfa-free medium. fm4-64 labeling showed typical bright apical staining in normally growing p. meyeri pollen tubes; this apical staining pattern differed from the v-formation pattern found in angiosperm pollen tubes. confocal microscopy revealed that exocytosis was greatly inhibited in the presence of bfa. in contrast, the overall uptake of fm4-64 dye was about 2-fold that in the control after bfa (5 mu g ml(-1)) treatment, revealing that bfa stimulated endocytosis in a manner opposite to the induced changes in exocytosis. transmission electron microscopic observation showed that the number of secretory vesicles at the apical zone dramatically decreased, together with the disappearance of paramural bodies, while the number of vacuoles and other larger organelles increased. an acid phosphatase assay confirmed that the addition of bfa significantly inhibited secretory pathways. importantly, fourier transform infrared microspectroscopy documented significant changes in the cell wall composition of pollen tubes growing in the presence of bfa. these results suggest that enhanced endocytosis, together with inhibited secretion, is responsible for the retarded growth of pollen tubes induced by bfa. |
| WOS关键词 | CELL-WALL PECTINS ; PLANT-CELLS ; VESICLE TRAFFICKING ; PROTEIN-SYNTHESIS ; ROOT-CELLS ; TIP GROWTH ; IN-VITRO ; MEMBRANE ; POLYSACCHARIDES ; ORGANIZATION |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000233903600012 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377452 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Lin, JX |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Photosynthesis & Mol Environm Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Univ Bonn, Inst Cellular & Mol Bot, Dept Plant Cell Biol, D-53115 Bonn, Germany 4.Slovak Acad Sci, Inst Plant Genet & Biotechnol, SK-95007 Nitra, Slovakia 5.Slovak Acad Sci, Inst Bot, SK-84223 Bratislava, Slovakia |
| 推荐引用方式 GB/T 7714 | Wang, QL,Kong, LG,Hao, HQ,et al. Effects of brefeldin a on pollen germination and tube growth. antagonistic effects on endocytosis and secretion[J]. Plant physiology,2005,139(4):1692-1703. |
| APA | Wang, QL.,Kong, LG.,Hao, HQ.,Wang, XH.,Lin, JX.,...&Baluska, F.(2005).Effects of brefeldin a on pollen germination and tube growth. antagonistic effects on endocytosis and secretion.Plant physiology,139(4),1692-1703. |
| MLA | Wang, QL,et al."Effects of brefeldin a on pollen germination and tube growth. antagonistic effects on endocytosis and secretion".Plant physiology 139.4(2005):1692-1703. |
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来源：中国科学院大学
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