Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c
文献类型：期刊论文
| 作者 | Zhao, HF; Wang, X; Zhang, GH |
| 刊名 | Febs letters
 |
| 出版日期 | 2005-02-28 |
| 卷号 | 579期号:6页码:1551-1556 |
| 关键词 | Lysosome Phospholipase c Osmotic sensitivity Calcium ion |
| ISSN号 | 0014-5793 |
| DOI | 10.1016/j.febslet.2005.01.061 |
| 通讯作者 | Zhang, gh() |
| 英文摘要 | Lysosomal disintegration is a crucial event for living cells, but mechanisms for the event are still unclear. in this study, we established that the cytosolic extracts could enhance lysosomal osmotic sensitivity and osmotically destabilize the lysosomes. the cytosol also caused the lysosomes to become more swollen in the hypotonic sucrose medium. the results indicate that the cytosol induced an osmotic shock to the lysosomes and an influx of water into the organelle. since the effects of cytosol on the lysosomes could be abolished by o-tricyclo[5.2. 1.0(2,6)]dec-9-yl dithiocarbonate potassium salt (d609), a specific inhibitor of cytosolic phospholipase c (plc), the plc might play an important role in the lysosomal osmotic destabilization. the activity of cytosolic plc and the extent of enzyme latency loss of the cytosol-treated lysosomes exhibited a similar biphasic dependence on the cytosolic ca2+ concentration. in addition, the cytosol did not osmotically destabilize the lysosomes until the cytosolic calcium ions rose above 100 nm. it suggests that the destabilization effect of cytosol on the lysosomes is ca2+-dependent. (c) 2005 federation of european biochemical societies. published by elsevier b.v. all rights reserved. |
| WOS关键词 | RAT-LIVER LYSOSOMES ; PHOSPHOLIPASE-C ; CELL-DEATH ; OSMOTIC SENSITIVITY ; CALCIUM ; APOPTOSIS ; MEMBRANE ; TRANSLOCATION ; ATP |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000227393700038 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377475 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, GH |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Univ Sci & Technol Beijing, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, HF,Wang, X,Zhang, GH. Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c[J]. Febs letters,2005,579(6):1551-1556. |
| APA | Zhao, HF,Wang, X,&Zhang, GH.(2005).Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c.Febs letters,579(6),1551-1556. |
| MLA | Zhao, HF,et al."Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c".Febs letters 579.6(2005):1551-1556. |
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来源：中国科学院大学
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