R-issr as a new tool for genomic fingerprinting, mapping, and gene tagging
文献类型：期刊论文
| 作者 | Ye, CJ; Yu, ZW; Kong, FN; Wu, SW; Wang, B |
| 刊名 | Plant molecular biology reporter
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 23期号:2页码:167-177 |
| 关键词 | Gene tagging Issr Rapd Sequencing gel Ssr |
| ISSN号 | 0735-9640 |
| 通讯作者 | Wang, b() |
| 英文摘要 | In the present study we propose and test the concept of r-issr, a new tool for genomic fingerprinting, mapping, and gene tagging. the concept is based on the fact that primers for inter-simple sequence repeat (issr) and random-amplified polymorphic dna (rapd) analysis elicit different genomic information, and the combined use of these 2 kinds of primers in the same polymerase chain reaction (pcr) reactions might reveal new genomic loci that could not be detected with either technique alone. the feasibility of this tool was first electronically simulated with sequence analysis software and arabidopsis chromosome sequence. next, different combinations of issr and rapd primers were applied in real pcr reactions to detect new genomic loci in 2 maize lines (q319 and 1145). sequencing gels were used to separate pcr products and showed good resolving ability in comparison with agarose gels. rapd primers could be successfully used with issr primers for the detection of new genomic loci and applied in a new way for genomic mapping, fingerprinting, and gene tagging. |
| WOS关键词 | TOMATO GENOME ; SEQUENCES ; DNA ; MICROSATELLITE ; REPEATS ; MARKERS ; L. ; POLYMORPHISMS ; AMPLIFICATION ; PCR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemical Research Methods ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000233144500007 |
| 出版者 | INT SOC PLANT MOLECULAR BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377514 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, B |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, CJ,Yu, ZW,Kong, FN,et al. R-issr as a new tool for genomic fingerprinting, mapping, and gene tagging[J]. Plant molecular biology reporter,2005,23(2):167-177. |
| APA | Ye, CJ,Yu, ZW,Kong, FN,Wu, SW,&Wang, B.(2005).R-issr as a new tool for genomic fingerprinting, mapping, and gene tagging.Plant molecular biology reporter,23(2),167-177. |
| MLA | Ye, CJ,et al."R-issr as a new tool for genomic fingerprinting, mapping, and gene tagging".Plant molecular biology reporter 23.2(2005):167-177. |
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来源：中国科学院大学
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