Purification and preliminary crystallographic studies of cutc, a novel copper homeostasis protein from shigella flexneri
文献类型：期刊论文
| 作者 | Zhu, YQ; Zhu, DY; Lu, HX; Yang, N; Li, GP; Wang, DC |
| 刊名 | Protein and peptide letters
 |
| 出版日期 | 2005-11-01 |
| 卷号 | 12期号:8页码:823-826 |
| 关键词 | Cutc Copper homeostasis protein Purification Crystallization Preliminary crystallographic studies Shigella flexneri |
| ISSN号 | 0929-8665 |
| 通讯作者 | Wang, dc() |
| 英文摘要 | Cutc is a novel copper homeostasis protein containing 248 amino acids. here we report the cloning, expression, purification, crystallization and preliminary x-ray crystallographic studies of cutc from shigella flexneri 2a. purification of cutc and its selenomethionine (semet) derivate were done using immobilized metal ion affinity chromatography, size-exclusion and ion-exchange chromatography. the purified proteins were crystallized using the hanging drop vapor diffusion method. the diffraction data for the native and semet cutc, respectively, have been collected with resolution of 1.7 angstrom and 2.1 angstrom. they belong to the space group c2221 and similar cell dimension. the native protein crystals have cell parameters: a=75.3267, b=97.6718, c=132.6910. |
| WOS关键词 | SIGNAL-TRANSDUCTION ; ESCHERICHIA-COLI ; NLPE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000232811400018 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377536 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, DC |
| 作者单位 | 1.Chinese Acad Sci, Ctr Struct & Mol Biol, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, YQ,Zhu, DY,Lu, HX,et al. Purification and preliminary crystallographic studies of cutc, a novel copper homeostasis protein from shigella flexneri[J]. Protein and peptide letters,2005,12(8):823-826. |
| APA | Zhu, YQ,Zhu, DY,Lu, HX,Yang, N,Li, GP,&Wang, DC.(2005).Purification and preliminary crystallographic studies of cutc, a novel copper homeostasis protein from shigella flexneri.Protein and peptide letters,12(8),823-826. |
| MLA | Zhu, YQ,et al."Purification and preliminary crystallographic studies of cutc, a novel copper homeostasis protein from shigella flexneri".Protein and peptide letters 12.8(2005):823-826. |
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来源：中国科学院大学
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