Cloning and heterologous expression of the nadph cytochrome p450 oxidoreductase genes from an industrial dicarboxylic acid-producing candida tropicalis
文献类型：期刊论文
| 作者 | He, F; Chen, YT |
| 刊名 | Yeast
 |
| 出版日期 | 2005-04-30 |
| 卷号 | 22期号:6页码:481-491 |
| 关键词 | Nadph cytochrome p450 oxidoreductase Cpr-a Cpr-b Cpr activity Ketoconazole Candida tropicalis Cpr mutant |
| ISSN号 | 0749-503X |
| DOI | 10.1002/yea.1227 |
| 通讯作者 | He, f(hefeng36@hotmail.com) |
| 英文摘要 | Nadph cytochrome p450 oxidoreductase (cpr) catalyses the transfer of electrons during p450-mediated oxidation, which plays an important role in the omega-oxidation pathway of candida tropicalis. two putative allelic genes, cpr-a and cpr-b, were cloned from the long chain dicarboxylic acid-producing candida tropicalis 1230, using cassette pcr methods. both the identified open reading frames predict the gene products of 679 amino acid residues. the deduced amino acid sequences of cpr-a and cpr-b are highly homologous to cpr genes from c. tropicalis atcc 750 and candida maltosa. both genes were individually expressed in a cpr mutant of saccharomyces cerevisiae with high cpr activities, in which only a small distinction was observed between recombinant cpr-a and cpr-b. both cpr-a and cpr-b contain one ctg codon, which codes for serine (amino acid 50) in c. tropicalis rather than universal leucine. a mutated cdna of cpr-a with a tcg codon instead of ctg codon was constructed and expressed, resulting in little increase in cpr activity. this indicates that the alteration of ser-50 has little effect on functional expression of cpr. furthermore, high ketoconazole sensitivity for the cpr mutant was complemented by heterologous expression of the cloned cpr-a or cpr-b. the sequences of cpr-a and cpr-b have been submitted to ddbj/embl/genebank databases under accession nos ay705446 and ay823228, respectively. copyright (c) 2005 john wiley & sons, ltd. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; MULTIGENE FAMILY ; N-ALKANE ; P450 OXIDOREDUCTASE ; YARROWIA-LIPOLYTICA ; CYP52 FAMILY ; C REDUCTASE ; IDENTIFICATION ; MEMBERS ; RESIDUES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 语种 | 英语 |
| WOS记录号 | WOS:000229252700006 |
| 出版者 | JOHN WILEY & SONS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377549 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, F |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Ctr Microbial Biotechnol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, F,Chen, YT. Cloning and heterologous expression of the nadph cytochrome p450 oxidoreductase genes from an industrial dicarboxylic acid-producing candida tropicalis[J]. Yeast,2005,22(6):481-491. |
| APA | He, F,&Chen, YT.(2005).Cloning and heterologous expression of the nadph cytochrome p450 oxidoreductase genes from an industrial dicarboxylic acid-producing candida tropicalis.Yeast,22(6),481-491. |
| MLA | He, F,et al."Cloning and heterologous expression of the nadph cytochrome p450 oxidoreductase genes from an industrial dicarboxylic acid-producing candida tropicalis".Yeast 22.6(2005):481-491. |
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来源：中国科学院大学
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