The study of high throughput maldi-tof genotyping assay
文献类型：期刊论文
| 作者 | Zhao, H; Wang, W; Zhang, QR; Gao, Y; Zhao, HB; Zhou, J; Lin, W; Zeng, CQ |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 32期号:7页码:667-672 |
| 关键词 | Single nucleotidepolymorphism (snp) Maldi-tof Genotyping |
| ISSN号 | 1000-3282 |
| 通讯作者 | Zhao, h(zhaoh@genomics.org.cn) |
| 英文摘要 | Maldi-tof is a simple and robust method for the analysis of single nucleotide polymorphism (snp), which combines proven high-fidelity enzymatic procedures and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. this technology is among the most promising snp typing methods for delivering accurate, effective, flexible and high throughput analysis of snps. "4-plex" genotyping protocol, which could detect 4 snps in one reaction, was performed in beijing genomics institute with accuracy of 99.55%. to learn the correlation between genotyping call rates and quality, nearly ten thousands of typing results ( "4-plex") were analyzed. significant positive correlation between call rate and the counts of conservative results were observed. snps with call rate lower than 82% have less conservative results, suggesting 82% was a cutoff to evaluate the genotyping results of maldi-tof assay. aiming to further improve genotyping throughput and reduce the cost, "mix 8-plex" and "double-spotting 8-plex" genotyping protocols were developed. for new protocols, pcr and primer extension reactions were still performed under reliable "4-plex" protocol. for "mix 8-plex" protocol, two sets of "4-plex" primer extension products were mixed and dispensed onto one spectrochip at the same time. as to "double-spotting 8-plex" protocol, two sets of "4-plex" products were consecutively dispensed onto one spectrochip. 32 snps were genotyped in 95 human dna samples to test the feasibility of these new protocols. the results showed that the performance of "mix 8-plex" was as good as "4-plex" protocol, while the performance of "double-spotting 8-plex" was poor. |
| WOS关键词 | SINGLE-NUCLEOTIDE POLYMORPHISMS ; MASS-SPECTROMETRY ; DISEASE ; SNPS ; DNA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000230606800015 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377553 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, H |
| 作者单位 | 1.Beijing Genom Inst, Beijing 101300, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100040, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, H,Wang, W,Zhang, QR,et al. The study of high throughput maldi-tof genotyping assay[J]. Progress in biochemistry and biophysics,2005,32(7):667-672. |
| APA | Zhao, H.,Wang, W.,Zhang, QR.,Gao, Y.,Zhao, HB.,...&Zeng, CQ.(2005).The study of high throughput maldi-tof genotyping assay.Progress in biochemistry and biophysics,32(7),667-672. |
| MLA | Zhao, H,et al."The study of high throughput maldi-tof genotyping assay".Progress in biochemistry and biophysics 32.7(2005):667-672. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。