Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film
文献类型：期刊论文
| 作者 | Liu, Y; Wang, MK; Zhao, F; Guo, ZH; Chen, HJ; Dong, SJ |
| 刊名 | Journal of electroanalytical chemistry
 |
| 出版日期 | 2005-07-15 |
| 卷号 | 581期号:1页码:1-10 |
| 关键词 | Microperoxidase-11 Carbon nanotubes Gold nanoparticles Electron transfer Electrocatalysis Nanohybrid film |
| ISSN号 | 0022-0728 |
| DOI | 10.1016/j.jelechem.2005.01.022 |
| 通讯作者 | Dong, sj() |
| 英文摘要 | The nanohybrid film (nhf) consisted of multi-wall carbon nanotubes (mwtns) with attached gold nanoparticles was used to immobilize protein. the nhf provides a favorable microenvironment for microperoxidase (mp-11) to perform direct electron transfer (det) at glassy carbon electrode. the nhf and mp-11 attached covalently were confirmed by transmission electron microscopy, atomic force microscopy and x-ray photoelectron spectroscopy. mp-11 immobilized on nhf surface exhibits a surface-controlled quasi-reversible cyclic voltammetric behavior. the electrochemical parameters such as apparent heterogeneous electron rate constant (k), formal potential (e-0') and the influence of ph on e-0' in the process were estimated by cyclic voltammetry. the mp-11 immobilized on the nhf retains its bioelectrocatalytic activity to the reduction of h2o2. the apparent michaelis-menten constant (k,,) and stability of the h2o2 response were investigated. moreover, it can catalyze o-2 through four-electron to water in ph 7.0 buffer solution. the kinetic constants for o-2 reduction (k(s) alpha n(alpha)) were obtained. the nhf to immobilize protein with det shows potential applicability of nanofabrication of biosensors and biofuel cells. (c) 2005 published by elsevier b.v. |
| WOS关键词 | GLASSY-CARBON ELECTRODE ; REDOX-ACTIVE MONOLAYERS ; BIOMEMBRANE-LIKE FILMS ; HORSERADISH-PEROXIDASE ; DIRECT ELECTROCHEMISTRY ; HYDROGEN-PEROXIDE ; COLLOIDAL GOLD ; CYTOCHROME-C ; PROTEIN ELECTROCHEMISTRY ; ENZYME ELECTRODE |
| WOS研究方向 | Chemistry ; Electrochemistry |
| WOS类目 | Chemistry, Analytical ; Electrochemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000230360900001 |
| 出版者 | ELSEVIER SCIENCE SA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377570 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dong, SJ |
| 作者单位 | Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanal Chem, Grad Sch, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Wang, MK,Zhao, F,et al. Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film[J]. Journal of electroanalytical chemistry,2005,581(1):1-10. |
| APA | Liu, Y,Wang, MK,Zhao, F,Guo, ZH,Chen, HJ,&Dong, SJ.(2005).Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film.Journal of electroanalytical chemistry,581(1),1-10. |
| MLA | Liu, Y,et al."Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film".Journal of electroanalytical chemistry 581.1(2005):1-10. |
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来源：中国科学院大学
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