Passage-restricted differentiation potential of mesenchymal stem cells into cardiomyocyte-like cells
文献类型：期刊论文
| 作者 | Zhang, FB; Li, L; Fang, B; Zhu, DL; Yang, HT; Gao, PJ |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-10-28 |
| 卷号 | 336期号:3页码:784-792 |
| 关键词 | Bone marrow mesenchymal stem cells Proliferation Differentiation Cardiomyocyte-like cells 5-azacytidine 5-bromo-2-deoxyuridine |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.08.177 |
| 通讯作者 | Yang, ht() |
| 英文摘要 | Mesenchymal stem cells (mscs) have limited ability to differentiate into cardiomyocytes and the factors affect this process are not fully understood. in this study, we investigated the passage (p)-related transdifferentiation potential of mscs into cardiomyocyte-like cells and its relationship to the proliferation ability. after 5-azacytidine treatment, only p4 but not p1 and p8 rat bone marrow mscs (rmscs) showed formation of myotube and expressed cardiomyocyte-associated markers. the growth property analysis showed p4 rmscs had a growth-arrest appearance, while p1 and p8 rmscs displayed an exponential growth pattern. when the rapid proliferation of p1 and p8 rmscs was inhibited by 5-bromo-2-deoxyuridine, a mitosis inhibitor, only p1, not p8 rmscs, differentiated into cardiomyocyte-like cells after 5-azacytidine treatment. these results demonstrate that the differentiation ability of rmscs into cardiomyocytes is in proliferation ability-dependent and passage-restricted patterns. these findings reveal a novel regulation on the transdifferentiation of mscs and provide useful information for exploiting the clinical therapeutic potential of mscs. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | MARROW STROMAL CELLS ; BONE-MARROW ; PROGENITOR CELLS ; EXPRESSION ; 5-AZACYTIDINE ; PROTEIN ; ORIGIN ; GROWTH ; NESTIN ; GENES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000232255000009 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377655 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, HT |
| 作者单位 | 1.Chinese Acad Sci, Mol Cardiol Lab, Shanghai 200025, Peoples R China 2.Chinese Acad Sci, Lab Vasc Biol, Inst Hlth Sci, SIBS, Shanghai 200025, Peoples R China 3.Shanghai Med Univ 2, CAS, Grad Sch, Shanghai 200025, Peoples R China 4.Shanghai Med Univ 2, Ruijin Hosp, Shanghai Inst Hypertens, Shanghai 200025, Peoples R China 5.Chinese Acad Sci, SBIS, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China 6.State Key Lab Med Genom, Shanghai Key Lab Vasc Biol, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, FB,Li, L,Fang, B,et al. Passage-restricted differentiation potential of mesenchymal stem cells into cardiomyocyte-like cells[J]. Biochemical and biophysical research communications,2005,336(3):784-792. |
| APA | Zhang, FB,Li, L,Fang, B,Zhu, DL,Yang, HT,&Gao, PJ.(2005).Passage-restricted differentiation potential of mesenchymal stem cells into cardiomyocyte-like cells.Biochemical and biophysical research communications,336(3),784-792. |
| MLA | Zhang, FB,et al."Passage-restricted differentiation potential of mesenchymal stem cells into cardiomyocyte-like cells".Biochemical and biophysical research communications 336.3(2005):784-792. |
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来源：中国科学院大学
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