Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration
文献类型：期刊论文
| 作者 | Li, YD; Shi, XL; Parry, G; Chen, LQ; Callahan, JA; Mazar, AP; Huang, MD |
| 刊名 | Protein and peptide letters
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 12期号:7页码:655-658 |
| 关键词 | Hydrogen peroxide Crystal optimization Upar Antibody |
| ISSN号 | 0929-8665 |
| 通讯作者 | Huang, md() |
| 英文摘要 | Optimization of protein crystal formation is often a necessary step leading to diffraction-quality crystals to enable collection of a full x-ray data set. typical protein crystal optimization involves screening different components, e.g., ph, precipitants, and additives of the precipitant solution. here we present an example using an inhibitory antibody of urokinase plasminogen activator receptor (upar) where such procedures did not yield diffracting crystals. in contrast, it was the treatment of the protein with hydrogen peroxide incubation and the protein concentration reduction that were found to be key factors in obtaining diffracting crystals. final crystals diffracted to 1.75 angstrom, and belong to orthorhombic p2(1)2(1)2(1) space group with unit cell parameters a=37.162 angstrom, b=84.474 angstrom, c=134.030 angstrom, and contain one molecule of fab fragment of anti-uro kinase receptor antibody in the asymmetric unit. |
| WOS关键词 | INTEGRIN FUNCTION ; CRYSTALLIZATION ; BINDING ; CANCER ; IDENTIFICATION ; VITRONECTIN ; MIGRATION ; THERAPY ; TARGET ; SITE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000231958900008 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377661 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, MD |
| 作者单位 | 1.Chinese Acad Sci, Fujian Inst Res Struct Matter, Grad Sch, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China 2.Attenuon LLC, San Diego, CA 92121 USA 3.Univ Alabama, Struct Biol Lab, Dept Chem, Grad Program Biotechnol, Huntsville, AL 35899 USA 4.Univ Alabama, Struct Biol Lab, Dept Chem, Grad Program Chem, Huntsville, AL 35899 USA 5.Univ Alabama, Struct Biol Lab, Dept Chem, Grad Program Mat Sci, Huntsville, AL 35899 USA |
| 推荐引用方式 GB/T 7714 | Li, YD,Shi, XL,Parry, G,et al. Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration[J]. Protein and peptide letters,2005,12(7):655-658. |
| APA | Li, YD.,Shi, XL.,Parry, G.,Chen, LQ.,Callahan, JA.,...&Huang, MD.(2005).Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration.Protein and peptide letters,12(7),655-658. |
| MLA | Li, YD,et al."Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration".Protein and peptide letters 12.7(2005):655-658. |
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来源：中国科学院大学
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