Characterization of a femo cofactor-deficient mofe protein from a nife-deleted strain (dj35) of azotobacter vinelandii
文献类型：期刊论文
| 作者 | Zhao, Y; Bian, SM; Zhang, CX; Zhou, HN; Wang, HP; Zhao, JF; Huang, JF |
| 刊名 | Chinese science bulletin
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 50期号:20页码:2305-2310 |
| 关键词 | Azotobaeter vinelandii Delta nife av1 Femoco P-cluster Epr |
| ISSN号 | 1001-6538 |
| DOI | 10.1360/982005-813 |
| 通讯作者 | Huang, jf() |
| 英文摘要 | A mofe protein (delta nije av1) with a purity of similar to 80% was purified from a nije-deleted mutant of azotobacter vinelandii dj35. compared with mofe protein purified from wild-type strain op (op av1), delta nife av1 had the same subunits composition, and had immune reaction with antibody to op av1, but its relative mobility in anaerobic native polyacrylamide gel electrophoresis (page) was a little larger than that of op av1. metal analysis showed that mo and fe contents of delta nife av1 both apparently decreased. when complemented with op fe protein, delta nife av1 had no c2h2-reduction activity, but it could be in vitro activated by femoco extracted from op av1. the circular dichroism (cd) spectrum of delta nife av1 at similar to 450 nm was similar to that of op av1, while the epr signal at g approximate to 3.7 was absolutely silent, and the signal intensities at g approximate to 4.3 and 2.0 decreased by 75% and 50%, respectively. the results indicated that delta nife av1 purified from dj35 was a femoco-deficient but p-cluster-containing mofe protein. |
| WOS关键词 | IRON-MOLYBDENUM COFACTOR ; KLEBSIELLA-PNEUMONIAE ; BIOSYNTHETIC GENES ; DEAE-CELLULOSE ; IN-VITRO ; NITROGENASE ; PURIFICATION ; MUTANT ; COMPLEX ; DINITROGENASE |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000234331800010 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377665 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, JF |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing 100080, Peoples R China 4.Fujian Normal Univ, Bioengn Coll, Fuzhou 360007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Y,Bian, SM,Zhang, CX,et al. Characterization of a femo cofactor-deficient mofe protein from a nife-deleted strain (dj35) of azotobacter vinelandii[J]. Chinese science bulletin,2005,50(20):2305-2310. |
| APA | Zhao, Y.,Bian, SM.,Zhang, CX.,Zhou, HN.,Wang, HP.,...&Huang, JF.(2005).Characterization of a femo cofactor-deficient mofe protein from a nife-deleted strain (dj35) of azotobacter vinelandii.Chinese science bulletin,50(20),2305-2310. |
| MLA | Zhao, Y,et al."Characterization of a femo cofactor-deficient mofe protein from a nife-deleted strain (dj35) of azotobacter vinelandii".Chinese science bulletin 50.20(2005):2305-2310. |
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来源：中国科学院大学
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