Expression of sars-coronavirus nucleocapsid protein in escherichia coli and lactococcus lactis for serodiagnosis and mucosal vaccination
文献类型：期刊论文
| 作者 | Pei, HD; Liu, JF; Cheng, Y; Sun, CM; Wang, C; Lu, YP; Ding, J; Zhou, J; Xiang, H |
| 刊名 | Applied microbiology and biotechnology
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 68期号:2页码:220-227 |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-004-1869-y |
| 通讯作者 | Xiang, h() |
| 英文摘要 | The nucleocapsid (n) protein of the severe acute respiratory syndrome (sars)-associated coronavirus (sars-cov) is an important antigen for the early diagnosis of sars and the development of vaccines. it was expressed in escherichia coli as a fusion with human glutathione s-transferase (hgst) and was confirmed by western blotting analysis. this recombinant n protein (hgst-n) was purified and used to measure the sars-cov n-specific antibody in the sera of eight sars patients by enzyme-linked immunosorbent assay. specific antibody response to this purified recombinant n protein was 100% positive in the sars patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. the sars-cov n protein was also expressed in lactococcus lactis in the cytoplasm or secreted into the medium. the n-producing strain mg1363/psecn and the purified hgst-n protein were respectively administered to mice, either orally or intranasally. results indicated that orally delivered mg1363/psecn induced significant n-specific igg in the sera. in conclusion, our work provides a novel strategy to produce the sars-cov n protein for serodiagnosis and for l. lactis-based mucosal vaccines. |
| WOS关键词 | ACUTE RESPIRATORY SYNDROME ; GLUTATHIONE-S-TRANSFERASE ; GENOME SEQUENCE ; GENE-EXPRESSION ; ACID BACTERIA ; CLONING |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000230844300010 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377734 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xiang, H |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chao Yang Hosp Affiliated Capital Univ Med Sci, Beijing 100020, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, HD,Liu, JF,Cheng, Y,et al. Expression of sars-coronavirus nucleocapsid protein in escherichia coli and lactococcus lactis for serodiagnosis and mucosal vaccination[J]. Applied microbiology and biotechnology,2005,68(2):220-227. |
| APA | Pei, HD.,Liu, JF.,Cheng, Y.,Sun, CM.,Wang, C.,...&Xiang, H.(2005).Expression of sars-coronavirus nucleocapsid protein in escherichia coli and lactococcus lactis for serodiagnosis and mucosal vaccination.Applied microbiology and biotechnology,68(2),220-227. |
| MLA | Pei, HD,et al."Expression of sars-coronavirus nucleocapsid protein in escherichia coli and lactococcus lactis for serodiagnosis and mucosal vaccination".Applied microbiology and biotechnology 68.2(2005):220-227. |
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来源：中国科学院大学
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