Detecting proton flux across chromatophores driven by f0f1-atpase using n-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt
文献类型：期刊论文
| 作者 | Cui, YB; Zhang, F; Yue, JC |
| 刊名 | Analytical biochemistry
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 344期号:1页码:102-107 |
| 关键词 | Proton flux F0f1-atpase Fluorescence probe Atp hydrolysis F-dhpe |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2005.06.027 |
| 通讯作者 | Yue, jc(yuejc@sun5.ibp.ac.cn) |
| 英文摘要 | N-(fluoreseein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sti-glycero-3-phosphoethanolamine, triethylammonium salt (f-dhpe) is a lipid fluorescence dye sensitive to ph changes and is used in this study for detecting proton flux through f0f1-atpase within chromatophores driven by atp hydrolysis. f-dhpe is easily labeled to the outer surface of chromatophores. in the range of ph 7.0 to 9.0, fluorescence intensity is sensitive to ph changes. the sensitivity is especially great in the range of ph 8.2 to 9.0, so ph 8.6 was chosen as the appropriate experimental condition. it is shown that added atp not only acts as a fluorescence quencher but also can be hydrolyzed by f0f1-atpase to pump protons into chromatophores, resulting in fluorescence restoration. a stimulator (naso3) and various types of inhibitors (nan3, 5'-adenylyl imidodiphosphate [amp pnp], and nn'-dicyclohexylcarbodiimide [dccd]) of f0f1 confirmed that fluorescence restoration is caused by atp-driven proton flux. when loaded with one antibody (anti-p antibody) or two antibodies (anti-p antibody and sheep to rabbit second antibody), f0f1-atpase exhibits lower proton pumping activities, as indicated by fluorescence restoration. the possible mechanism of the inhibition of antibodies on proton pumping activity is discussed. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | ATP SYNTHASE ; RHODOSPIRILLUM-RUBRUM ; MOLECULAR MACHINE ; MOTOR ; F-1-ATPASE ; VESICLES ; SUBUNIT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000231544500012 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377771 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yue, JC |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, YB,Zhang, F,Yue, JC. Detecting proton flux across chromatophores driven by f0f1-atpase using n-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt[J]. Analytical biochemistry,2005,344(1):102-107. |
| APA | Cui, YB,Zhang, F,&Yue, JC.(2005).Detecting proton flux across chromatophores driven by f0f1-atpase using n-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt.Analytical biochemistry,344(1),102-107. |
| MLA | Cui, YB,et al."Detecting proton flux across chromatophores driven by f0f1-atpase using n-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt".Analytical biochemistry 344.1(2005):102-107. |
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来源：中国科学院大学
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