Human protein tau represses dna replication in vitro
文献类型：期刊论文
| 作者 | Li, W; Wang, XS; Qu, MH; Liu, Y; He, RQ |
| 刊名 | Biochimica et biophysica acta-general subjects
 |
| 出版日期 | 2005-11-30 |
| 卷号 | 1726期号:3页码:280-286 |
| 关键词 | Protein tau Dna replication Transcription Repression Pcr |
| ISSN号 | 0304-4165 |
| DOI | 10.1016/j.bbagen.2005.08.014 |
| 通讯作者 | He, rq() |
| 英文摘要 | Here, in the experiments of both pcr and real-time pcr, a repression of dna amplification was observed in the presence of protein tau. furthermore, a strong repression appeared when an in vitro dna replication assay was performed at the physiological temperature (37 degrees c). the incorporation of dntp was markedly decreased to similar to 12% of control by the presence of tau23 and to similar to 15% by tau40. in the competitive experiments, the pcr product could be restored when the competitor dna was added, indicating that the association of tau with the template gave rise to the repression. however, tau did not repress the yield of rna in transcription, suggesting that tau was replaced or ejected from the template by the elongating t7 rna polymerase. (c) 2005 elsevier b.v. all rights reserved. |
| WOS关键词 | MICROTUBULE-ASSOCIATED PROTEIN ; SINGLE-STRANDED-DNA ; CELLS ; PHOSPHORYLATION ; POLYMERASE ; NUCLEOSOME ; MICROSCOPY ; NUCLEAR ; BINDS ; ASSAY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000233709400007 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377873 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, RQ |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, W,Wang, XS,Qu, MH,et al. Human protein tau represses dna replication in vitro[J]. Biochimica et biophysica acta-general subjects,2005,1726(3):280-286. |
| APA | Li, W,Wang, XS,Qu, MH,Liu, Y,&He, RQ.(2005).Human protein tau represses dna replication in vitro.Biochimica et biophysica acta-general subjects,1726(3),280-286. |
| MLA | Li, W,et al."Human protein tau represses dna replication in vitro".Biochimica et biophysica acta-general subjects 1726.3(2005):280-286. |
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来源：中国科学院大学
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