Imaging dna molecules on mica surface by atomic force microscopy in air and in liquid
文献类型：期刊论文
| 作者 | Liu, ZG; Li, Z; Zhou, HL; Wei, G; Song, YH; Wang, L |
| 刊名 | Microscopy research and technique
 |
| 出版日期 | 2005-03-01 |
| 卷号 | 66期号:4页码:179-185 |
| 关键词 | Dna Atomic force microscopy (afm) Mica Divalent cations 3-aminopropyltriethoxysilane (aptes) |
| ISSN号 | 1059-910X |
| DOI | 10.1002/jemt.20156 |
| 通讯作者 | Li, z(zli@ciac.jl.cn) |
| 英文摘要 | Dna molecules immobilized on mica surface by various methods have been observed by atomic force microscopy both in air and in liquid. divalent cations and 3-aminopropyltriethoxysilane (aptes) modified mica surface have been used to immobilize the dna molecules. optimal dna and divalent cations concentration for afm imaging are presented. among the different methods of modifying mica surface with aptes, the water solution modifying method appears to get the best results. when using high dna concentration for afm imaging, dna networks can be formed. a simple method to extend long dna molecules is demonstrated. the optimal imaging conditions and afm operating techniques are discussed. different dna immobilizing methods have been compared and evaluated. (c) 2005 wiley-liss, inc. |
| WOS关键词 | PLASMID DNA ; COMPLEXES ; BINDING ; AFM ; VISUALIZATION ; CONDENSATION ; WATER ; GOLD |
| WOS研究方向 | Anatomy & Morphology ; Life Sciences & Biomedicine - Other Topics ; Microscopy |
| WOS类目 | Anatomy & Morphology ; Biology ; Microscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000229737700003 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377874 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Z |
| 作者单位 | Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Grad Sch, Changchun 130022, Jilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, ZG,Li, Z,Zhou, HL,et al. Imaging dna molecules on mica surface by atomic force microscopy in air and in liquid[J]. Microscopy research and technique,2005,66(4):179-185. |
| APA | Liu, ZG,Li, Z,Zhou, HL,Wei, G,Song, YH,&Wang, L.(2005).Imaging dna molecules on mica surface by atomic force microscopy in air and in liquid.Microscopy research and technique,66(4),179-185. |
| MLA | Liu, ZG,et al."Imaging dna molecules on mica surface by atomic force microscopy in air and in liquid".Microscopy research and technique 66.4(2005):179-185. |
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来源：中国科学院大学
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