Genetic diversity among silkworm (bombyx mori l., lep., bombycidae) germplasms revealed by microsatellites
文献类型：期刊论文
| 作者 | Li, MW; Shen, L; Xu, AY; Miao, XX; Hou, CX; Sun, PJ; Zhang, YH; Huang, YP |
| 刊名 | Genome
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 48期号:5页码:802-810 |
| 关键词 | Silkworm Bombyx mori l. Microsatellites Simple sequence repeat (ssr) Genetic diversity |
| ISSN号 | 0831-2796 |
| 通讯作者 | Huang, yp() |
| 英文摘要 | To determine genetic relationships among strains of silkworm, bombyx mori l., 31 strains with different origins, number of generations per year, number of molts per generation, and morphological characters were studied using simple sequence repeat (ssr) markers. twenty-six primer pairs flanking microsatellite sequences in the silkworm genome were assayed. all were polymorphic and unambiguously separated silkworm strains from each other. a total of 188 alleles were detected with a mean value of 7.2 alleles/locus (range 2-17). the average heterozygosity value for each ssr locus ranged from 0 to 0.60, and the highest one was 0.96 (1710516 in 4013). the mean polymorphism index content (pic) was 0.66 (range 0.12-0.89). unweighted pair group method with arithmetic means (upgma) cluster analysis of nei's genetic distance grouped silkworm strains based on their origin. seven major ecotypic silkworm groups were analyzed. principal components analysis (pca) for ssr data support their upgma clustering. the results indicated that ssr markers are an efficient tool for fingerprinting cultivars and conducting genetic-diversity studies in the silkworm. |
| WOS关键词 | FRAGMENT-LENGTH-POLYMORPHISMS ; MOLECULAR MARKERS ; LINKAGE MAP ; SSR MARKERS ; DNA MARKERS ; IDENTIFICATION ; DISTANCES ; TRAITS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000234082400005 |
| 出版者 | NATL RESEARCH COUNCIL CANADA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378022 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, YP |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, MW,Shen, L,Xu, AY,et al. Genetic diversity among silkworm (bombyx mori l., lep., bombycidae) germplasms revealed by microsatellites[J]. Genome,2005,48(5):802-810. |
| APA | Li, MW.,Shen, L.,Xu, AY.,Miao, XX.,Hou, CX.,...&Huang, YP.(2005).Genetic diversity among silkworm (bombyx mori l., lep., bombycidae) germplasms revealed by microsatellites.Genome,48(5),802-810. |
| MLA | Li, MW,et al."Genetic diversity among silkworm (bombyx mori l., lep., bombycidae) germplasms revealed by microsatellites".Genome 48.5(2005):802-810. |
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来源：中国科学院大学
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