Complex assembly, crystallization and preliminary x- ray crystallographic studies of rhesus macaque mhc mamu-a*01 complexed with an immunodominant siv-gag nonapeptide
文献类型：期刊论文
| 作者 | Chu, FL; Lou, ZY; Gao, B; Bell, JI; Rao, ZH; Gao, GF |
| 刊名 | Acta crystallographica section f-structural biology and crystallization communications
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 61页码:614-616 |
| ISSN号 | 1744-3091 |
| 通讯作者 | Gao, gf(gaof@im.ac.cn) |
| 英文摘要 | Simian immunodeficiency virus (siv) infection in rhesus macaques has been used as the best model for the study of human immunodeficiency virus (hiv) infection in humans, especially in the cytotoxic t-lymphocyte (ctl) response. however, the structure of rhesus macaque (or any other monkey model) major histocompatibility complex class i (mhc i) presenting a specific peptide (the ligand for ctl) has not yet been elucidated. here, using in vitro refolding, the preparation of the complex of the rhesus macaque mhc i allele (mamu-a* 01) with human beta(2)m and an immunodominant peptide, ctpydinqm (gag_cm9), derived from siv gag protein is reported. the complex ( 45 kda) was crystallized; the crystal belongs to space group i422, with unit-cell parameters a = b = 183.8, c = 155.2 angstrom. the crystal contains two molecules in the asymmetric unit and diffracts x-rays to 2.8 angstrom resolution. the structure is being solved by molecular replacement and this is the first attempt to determined the crystal structure of a peptide-nonhuman primate mhc complex. |
| WOS关键词 | SIMIAN IMMUNODEFICIENCY VIRUS ; BINDING ; PEPTIDES ; SEQUENCE ; EPITOPE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 语种 | 英语 |
| WOS记录号 | WOS:000232319200021 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378029 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, GF |
| 作者单位 | 1.Chinese Acad Sci, Ctr Mol Immunol, Inst Microbiol, Lab Mol Immunol & Mol Virol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Tsinghua Univ, Struct Biol Lab, Beijing 100084, Peoples R China 4.UCL, Inst Child Hlth, London WC1N 1EH, England 5.Univ Oxford, Nuffield Dept Clin Med, Oxford OX3 9DU, England |
| 推荐引用方式 GB/T 7714 | Chu, FL,Lou, ZY,Gao, B,et al. Complex assembly, crystallization and preliminary x- ray crystallographic studies of rhesus macaque mhc mamu-a*01 complexed with an immunodominant siv-gag nonapeptide[J]. Acta crystallographica section f-structural biology and crystallization communications,2005,61:614-616. |
| APA | Chu, FL,Lou, ZY,Gao, B,Bell, JI,Rao, ZH,&Gao, GF.(2005).Complex assembly, crystallization and preliminary x- ray crystallographic studies of rhesus macaque mhc mamu-a*01 complexed with an immunodominant siv-gag nonapeptide.Acta crystallographica section f-structural biology and crystallization communications,61,614-616. |
| MLA | Chu, FL,et al."Complex assembly, crystallization and preliminary x- ray crystallographic studies of rhesus macaque mhc mamu-a*01 complexed with an immunodominant siv-gag nonapeptide".Acta crystallographica section f-structural biology and crystallization communications 61(2005):614-616. |
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来源：中国科学院大学
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