Generation of murine ctl by a hepatitis b virus-specific peptide and evaluation of the adjuvant effect of heat shock protein glycoprotein 96 and its terminal fragments
文献类型：期刊论文
| 作者 | Li, HT; Zhou, MH; Han, JL; Zhu, XD; Dong, T; Gao, GF; Tien, P |
| 刊名 | Journal of immunology
 |
| 出版日期 | 2005 |
| 卷号 | 174期号:1页码:195-204 |
| ISSN号 | 0022-1767 |
| 通讯作者 | Tien, p() |
| 英文摘要 | Previously, we reported that a 7-mer hla-a11-restricted peptide (yvntnmg) of hepatitis b virus (hbv) core ag (hbcag(88-94)) was associated with heat shock protein (hsp) gp96 in liver tissues of patients with hbv-induced hepatocellular carcinoma (hcc). this peptide is highly homologous to a human hla-a11-restricted 9-mer peptide (yvnvnmglk), and to a mouse h-2-k-d- restricted 9-mer peptide (syvntnmgl). to further characterize its immunogenicity, balb/c mice were vaccinated with the hbv 7-mer peptide. it was found that a specific ctl response was induced by the 7-mer peptide, although the response was similar to50 % of that induced by the mouse h-2-k-d-restricted 9-mer peptide, as detected by elispot, tetramer, and cr-51 release assays. to evaluate the adjuvant effect of hsp gp96, mice were coimmunized with gp96 and the 9-mer peptide, and a significant adjuvant effect was observed with gp96. to further determine whether the immune effect of gp96 was dependent on peptide binding, the n- and c-terminal fragments of gp96, which are believed to contain the putative peptide-binding domain, were cloned and expressed in escherichia coli. ctl assays indicated that only the n-terminal fragment, but not the c-terminal fragment, was able to produce the adjuvant effect. these results clearly demonstrated the potential of using gp96 or its n-terminal fragment as a possible adjuvant to augment ctl response against hbv infection and hcc. |
| WOS关键词 | CYTOTOXIC T-LYMPHOCYTES ; ANTIGEN-PRESENTING CELLS ; TUMOR-IMMUNITY ; IN-VIVO ; CHAPERONED PEPTIDES ; GP96 PREPARATIONS ; DENDRITIC CELLS ; CANCER-PATIENTS ; BINDING-SITE ; VACCINES |
| WOS研究方向 | Immunology |
| WOS类目 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000225852900023 |
| 出版者 | AMER ASSOC IMMUNOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378094 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tien, P |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Dept Mol Virol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Oxford OX1 2JD, England |
| 推荐引用方式 GB/T 7714 | Li, HT,Zhou, MH,Han, JL,et al. Generation of murine ctl by a hepatitis b virus-specific peptide and evaluation of the adjuvant effect of heat shock protein glycoprotein 96 and its terminal fragments[J]. Journal of immunology,2005,174(1):195-204. |
| APA | Li, HT.,Zhou, MH.,Han, JL.,Zhu, XD.,Dong, T.,...&Tien, P.(2005).Generation of murine ctl by a hepatitis b virus-specific peptide and evaluation of the adjuvant effect of heat shock protein glycoprotein 96 and its terminal fragments.Journal of immunology,174(1),195-204. |
| MLA | Li, HT,et al."Generation of murine ctl by a hepatitis b virus-specific peptide and evaluation of the adjuvant effect of heat shock protein glycoprotein 96 and its terminal fragments".Journal of immunology 174.1(2005):195-204. |
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来源：中国科学院大学
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