Enhancement of humoral immune responses to hbsag by heat shock protein gp96 and its n-terminal fragment in mice
文献类型：期刊论文
| 作者 | Li, Hong-Tao1; Yan, Jia-Bin1; Li, Jing2; Zhou, Ming-Hai1; Zhu, Xiao-Dong1; Zhang, Yu-Xia1; Tien, Po1 |
| 刊名 | World journal of gastroenterology
 |
| 出版日期 | 2005-05-21 |
| 卷号 | 11期号:19页码:2858-2863 |
| 关键词 | Heat shock protein Gp96 n-terminal fragment Hbv Hepatitis b virus surface antigen (hbsag) Vaccine |
| ISSN号 | 1007-9327 |
| 通讯作者 | Tien, po(tienpo@sun.im.ac.cn) |
| 英文摘要 | Aim: most studies on the immune effect of gp96 were focused on its enhancement of ctls. it is interesting to know whether gp96 could influence the humoral immune response, and whether the recombinant n-terminal fragment of gp96 could substitute native gp96 to stimulate the immune system. methods: gp96 isolated from livers of normal mice and its n-terminal fragment (amino acid 22-355) expressed in e coli were used for immunization of balb/c mice. eight groups of mice received one of the following regiments subcutaneously in 100 mu l phosphate buffered saline (pbs) at an interval of 3 wk. group 1: pbs only; group 2: gp96 only; group 3: n-terminal fragment only; group 4: hbsag only; group 5: hbsag+gp96; group 6: hbsag+n-terminal fragment; group 7: hbsag+incomplete freud's adjuvant; group 8: hbsag+n-terminal fragment (95 degrees c heated for 30 min). serum anti-hbsag antibody levels were assayed by elisa. ctl responses in splenocytes were analyzed by elispot after the last vaccination. results: the average titer of serum anti-hbsag antibody in the mice immunized with hbsag together with gp96 or its n-terminal fragment were much higher than those immunized with hbsag alone detected by elisa. the cellular immune response of the mice immunized with hbsag together with gp96 or its n-terminal fragment was not different with those immunized with hbsag alone measured by elispot assay. conclusion: gp96 or its n-terminal fragment greatly improved humoral immune response induced by hbsag, but failed to enhance the ctl response, which demonstrated the potential of using gp96 or its n-terminal fragment as a possible adjuvant to augment humoral immune response against hbv infection. (c) 2005 the wjg press and elsevier inc. all rights reserved. |
| WOS研究方向 | Gastroenterology & Hepatology |
| WOS类目 | Gastroenterology & Hepatology |
| 语种 | 英语 |
| WOS记录号 | WOS:000208098400002 |
| 出版者 | BAISHIDENG PUBL GRP CO LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378117 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tien, Po |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Dept Mol Virol, Grad Sch, Beijing 100080, Peoples R China 2.Natl Vaccine & Serum Inst, Hepatitis Dept B, Beijing 100024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hong-Tao,Yan, Jia-Bin,Li, Jing,et al. Enhancement of humoral immune responses to hbsag by heat shock protein gp96 and its n-terminal fragment in mice[J]. World journal of gastroenterology,2005,11(19):2858-2863. |
| APA | Li, Hong-Tao.,Yan, Jia-Bin.,Li, Jing.,Zhou, Ming-Hai.,Zhu, Xiao-Dong.,...&Tien, Po.(2005).Enhancement of humoral immune responses to hbsag by heat shock protein gp96 and its n-terminal fragment in mice.World journal of gastroenterology,11(19),2858-2863. |
| MLA | Li, Hong-Tao,et al."Enhancement of humoral immune responses to hbsag by heat shock protein gp96 and its n-terminal fragment in mice".World journal of gastroenterology 11.19(2005):2858-2863. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。