Rapid determination of diterpenoids in andrographis paniculata by microemulsion electrokinetic capillary chromatography with short-end injection
文献类型：期刊论文
| 作者 | Zhao, YF; Ming, YF; Zhang, HL; Luo, XP; Chen, LR; Li, YM |
| 刊名 | Chromatographia
 |
| 出版日期 | 2005-12-01 |
| 卷号 | 62期号:11-12页码:611-615 |
| 关键词 | Capillary electropkoresis Microemulsion electrokinetic chromatography Skort-end injection Diterpenoids Andrographis paniculoto |
| ISSN号 | 0009-5893 |
| DOI | 10.1365/s10337-005-0676-7 |
| 通讯作者 | Chen, lr() |
| 英文摘要 | A rapid, simple, and reliable method has been developed for routine capillary electrophoretic analysis of two diterpenoids, andrographolide and dehydroandrographolide, in androgrophis paniculata. for this system, using ethyl acetate oil-based microemulsion electrokinetic chromatography (meekc) and the short-end injection technique, analysis was complete in less than 2.5 min. in method validation the relative standard deviations of migration time and peak area of the two constituents were, respectively, 0.54% and 1.70% for andrographolide and 0.45% and 2.11 % for dehydroandrographolide. regression equations revealed linear relationships (correlation coefficients 0.9994 for andrographolide and 0.9993 for dehydroandrographolide) between peak area and concentration. the effects of buffer ph, borate concentration, sds concentration, co-surfactant type and concentration, injection time, and running potential were systematically investigated. the method can be successfully implemented in routine quality-control testing. |
| WOS关键词 | HEPATOPROTECTIVE DITERPENOIDS ; BIOACTIVE DITERPENOIDS ; PRODUCTS ; SEPARATION ; HPLC ; NEOANDROGRAPHOLIDE ; PERFORMANCE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000234235400009 |
| 出版者 | VIEWEG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378134 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, LR |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, YF,Ming, YF,Zhang, HL,et al. Rapid determination of diterpenoids in andrographis paniculata by microemulsion electrokinetic capillary chromatography with short-end injection[J]. Chromatographia,2005,62(11-12):611-615. |
| APA | Zhao, YF,Ming, YF,Zhang, HL,Luo, XP,Chen, LR,&Li, YM.(2005).Rapid determination of diterpenoids in andrographis paniculata by microemulsion electrokinetic capillary chromatography with short-end injection.Chromatographia,62(11-12),611-615. |
| MLA | Zhao, YF,et al."Rapid determination of diterpenoids in andrographis paniculata by microemulsion electrokinetic capillary chromatography with short-end injection".Chromatographia 62.11-12(2005):611-615. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。