The expression of tgf-beta 1 in uterus and placenta of pregnant rat and its regulation by ifn gamma
文献类型：期刊论文
| 作者 | Liu, ML; Peng, JP; Sun, QH; Yang, Y; Xia, HF |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2005-05-01 |
| 卷号 | 32期号:5页码:413-420 |
| 关键词 | Pregnant rat Placenta Uterus Tgf-beta 1 Ifn gamma |
| ISSN号 | 1000-3282 |
| 通讯作者 | Peng, jp(pengjp@ioz.ac.cn) |
| 英文摘要 | To investigate the expression change and location of transforming growth factor-beta 1 (tgf-beta 1) at uterus and placenta in the course of pregnancy and the effects of interferon gamma (ifn gamma) on its expression, the expression of tgf-beta 1 was examined in uterus and placenta in different stages of gestation by methods of rt-pcr, western blot, and immunohistochemistry respectively. and rt-pcr was used to analyze the expression of tgf-beta 1 mrna after injecting different dose of ifn gamma. experimental results indicated that the expression of tgf-beta 1 mrna and tgf-beta 1 protein were detected in uterus and placenta in every stage of gestation. the analysis of semie-quantitative rt-pcr that showed the expression of tgf-beta 1 mrna was increasing gradually at uterus during the early stage of gestation (d1-d9). the expression of tgf-beta 1 in pre-implantation (d4) period was lower than that in implantation (d6) period and post-implantation (d9) period. the expression in post-implantation (d9) period is highest at uterus during gestation. with the process of gestation, the expression of tgf-beta 1 decreased significantly at uterus during mid-stage (d 15) and late stage (d 19) of gestation. the expression of tgf-beta 1 at placenta kept increasing in the course of pregnancy. the expression of tgf-beta 1 at mid-stage placenta (d15) was significantly higher (p < 0.01) compared with that at early stage placenta (d9) and the expression of tgf-beta 1 at late-stage placenta (d19) was significantly higher (p < 0.01) compared with that at mid-stage placenta (d15). the analysis of immunohistochemistry indicated tgf-beta 1 mostly expressed at decidua during early stage of gestation and at trophoblast during mid-stage and late stage of gestation. after injecting exceeding normal concentration ifn gamma by vaginal muscular, the expression of tgf-beta 1 was downregulated at uterus and placenta of pregnant rat. the results suggested that the expression of tgf-beta 1 was dynamic and spatio-temporal characteristics in uterus and placenta during gestation. ifn gamma affected the expression of tgf-beta 1 at uterus and placenta in the course of pregnancy. |
| WOS关键词 | CLASS-II EXPRESSION ; GROWTH-FACTOR ; INTERFERON-GAMMA ; EPITHELIAL-CELLS ; TROPHOBLAST CELL ; TGF-BETA ; MHC ; MACROPHAGES ; MOUSE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000229250000006 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378170 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Peng, JP |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Dept Biol, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, ML,Peng, JP,Sun, QH,et al. The expression of tgf-beta 1 in uterus and placenta of pregnant rat and its regulation by ifn gamma[J]. Progress in biochemistry and biophysics,2005,32(5):413-420. |
| APA | Liu, ML,Peng, JP,Sun, QH,Yang, Y,&Xia, HF.(2005).The expression of tgf-beta 1 in uterus and placenta of pregnant rat and its regulation by ifn gamma.Progress in biochemistry and biophysics,32(5),413-420. |
| MLA | Liu, ML,et al."The expression of tgf-beta 1 in uterus and placenta of pregnant rat and its regulation by ifn gamma".Progress in biochemistry and biophysics 32.5(2005):413-420. |
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来源：中国科学院大学
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