Isolation and expression analysis of salt up-regulated ests in upland rice using pcr-based subtractive suppression hybridization method
文献类型：期刊论文
| 作者 | Wu, YR; Wang, QY; Ma, YM; Chu, CC |
| 刊名 | Plant science
 |
| 出版日期 | 2005-03-01 |
| 卷号 | 168期号:3页码:847-853 |
| 关键词 | Salt stress Subtractive suppression hybridization Upland rice |
| ISSN号 | 0168-9452 |
| DOI | 10.1016/j.plantsci.2004.10.020 |
| 通讯作者 | Chu, cc() |
| 英文摘要 | Salt stress adversely affects the growth, development, and production of rice plants. to understand the molecular basis of salt stress response, the salt tolerant upland rice variety iapar 9 was used to identify the genes differentially expressed in nacl-treated roots and nontreated ones by subtractive suppression hybridization method. after subtraction the cdna fragments were cloned and a total of 94 unique clones were identified which were up-regulated under salt stress. database search of the sequenced clones showed that 31 unique clones were homologous to stress response genes reported previously. the expression patterns of several clones have been further analyzed via northern blot, suggesting that some of them may be involved in stress responses. (c) 2004 elsevier ireland ltd. all rights reserved. |
| WOS关键词 | ORYZA-SATIVA L. ; GENE-EXPRESSION ; PLANT-RESPONSES ; DRAFT SEQUENCE ; OSMOTIC-STRESS ; PROTEIN GENE ; INDUCTION ; TOLERANCE ; PATHWAYS ; DROUGHT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000227205900031 |
| 出版者 | ELSEVIER SCI IRELAND LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378213 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chu, CC |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Plant Gene Res Ctr, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, YR,Wang, QY,Ma, YM,et al. Isolation and expression analysis of salt up-regulated ests in upland rice using pcr-based subtractive suppression hybridization method[J]. Plant science,2005,168(3):847-853. |
| APA | Wu, YR,Wang, QY,Ma, YM,&Chu, CC.(2005).Isolation and expression analysis of salt up-regulated ests in upland rice using pcr-based subtractive suppression hybridization method.Plant science,168(3),847-853. |
| MLA | Wu, YR,et al."Isolation and expression analysis of salt up-regulated ests in upland rice using pcr-based subtractive suppression hybridization method".Plant science 168.3(2005):847-853. |
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来源：中国科学院大学
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