Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi
文献类型：期刊论文
| 作者 | Zhao, Xin1,3; Kong, Xiangli2; Hua, Yanyan1; Feng, Bin2; Zhao, Zongbao (Kent)1 |
| 刊名 | european journal of lipid science and technology
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 110期号:5页码:405-412 |
| 关键词 | co-fermentation Lipomyces starkeyi microbial oil oleaginous yeast response surface methodology |
| 产权排序 | 1;1 |
| 通讯作者 | 赵宗保 |
| 英文摘要 | co-fermentation of lignocellulose-based carbohydrates is a potential solution to improve the economics of microbial lipid production. in the present paper, experiments were performed to optimize the media composition for lipid production by the oleaginous yeast lipomyces starkeyi as 2.1560 through co-fermentation of glucose and xylose (2 : 1 wt/wt). statistical screening of nine media variables was performed by a plackett-burman design. three factors, namely mixed sugar, yeast extract and feso4, were found as significant components influencing cellular lipid accumulation. further optimization was carried out using a box-behnken factorial design to study the effects of these three variables on lipid production. a mathematical model with the r-2 value at 96.66% was developed to show the effect of each medium composition and their interactions on the lipid production. the model estimated that a maximal lipid content of 61.0 wt-% could be obtained when the concentrations of mixed sugar, yeast extract and feso4 were at 73.3 g/l (glucose 48.9 g/l, xylose 24.4 g/l), 7.9 g/l and 4.0 mg/l, respectively. the predicted value was in good accordance with the experimental data of 61.5%. compared with the initial media, the optimized media gave 1.59-fold and 2.03-fold increases for lipid content and lipid productivity, respectively. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | food science & technology ; nutrition & dietetics |
| 研究领域[WOS] | food science & technology ; nutrition & dietetics |
| 关键词[WOS] | response-surface methodology ; stage continuous-culture ; fed-batch culture ; yarrowia-lipolytica ; cryptococcus curvatus ; apiotrichum-curvatum ; biodiesel production ; density cultivation ; microorganisms ; accumulation |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000256537000005 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101145]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Liaoning Normal Univ, Coll Life Sci, Dalian, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xin,Kong, Xiangli,Hua, Yanyan,et al. Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi[J]. european journal of lipid science and technology,2008,110(5):405-412. |
| APA | Zhao, Xin,Kong, Xiangli,Hua, Yanyan,Feng, Bin,&Zhao, Zongbao .(2008).Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi.european journal of lipid science and technology,110(5),405-412. |
| MLA | Zhao, Xin,et al."Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi".european journal of lipid science and technology 110.5(2008):405-412. |
入库方式： OAI收割
来源：大连化学物理研究所
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