Design of recombinant protein-based sars-cov entry inhibitors targeting the heptad-repeat regions of the spike protein s2 domain
文献类型：期刊论文
| 作者 | Ni, L; Zhu, JQ; Zhang, JJ; Yan, M; Gao, GF; Tien, P |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-04-29 |
| 卷号 | 330期号:1页码:39-45 |
| 关键词 | Design Sars-cov Protein inhibitors Heptad-repeat regions Spike protein Pseudotyped virus |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.02.117 |
| 通讯作者 | Gao, gf() |
| 英文摘要 | Entry of sars-cov into a target cell is initiated by binding of the si domain of spike protein to a receptor, followed by conformational changes of the spike protein s2 domain, resulting in the formation of a six-helix bundle by the heptad-repeat (hr1 and hr2) regions. our previous studies have demonstrated that peptides derived from hr2 region could inhibit sars-cov entry. however, synthesis of these peptides is at high cost. in this study, we designed two recombinant proteins, one containing two hr1 and one hr2 peptides (denoted hr121), and the other consisting of two hr2 and one hr1 peptides (designated hr212). these two proteins could be easily purified with the low cost of production, exhibiting high stability and potent inhibitory activity on entry of the hiv/sars pseudoviruses with ic50 values of 4.13 and 0.95 mu m, respectively. these features suggest that hr121 and hr212 can serve as potent inhibitors of sars-cov entry. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | ACUTE RESPIRATORY SYNDROME ; VIRAL MEMBRANE-FUSION ; SYNDROME CORONAVIRUS ; 6-HELIX BUNDLE ; EXPRESSION CLONING ; VIRUS ; CORE ; IDENTIFICATION ; GLYCOPROTEIN ; MECHANISM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000228253700007 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378236 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, GF |
| 作者单位 | 1.Chinese Acad Sci, Dept Mol Virol, Inst Microbiol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ni, L,Zhu, JQ,Zhang, JJ,et al. Design of recombinant protein-based sars-cov entry inhibitors targeting the heptad-repeat regions of the spike protein s2 domain[J]. Biochemical and biophysical research communications,2005,330(1):39-45. |
| APA | Ni, L,Zhu, JQ,Zhang, JJ,Yan, M,Gao, GF,&Tien, P.(2005).Design of recombinant protein-based sars-cov entry inhibitors targeting the heptad-repeat regions of the spike protein s2 domain.Biochemical and biophysical research communications,330(1),39-45. |
| MLA | Ni, L,et al."Design of recombinant protein-based sars-cov entry inhibitors targeting the heptad-repeat regions of the spike protein s2 domain".Biochemical and biophysical research communications 330.1(2005):39-45. |
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来源：中国科学院大学
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