1.88 angstrom crystal structure of the c domain of hcyp33: a novel domain of peptidyl-prolyl cis-trans isomerase
文献类型：期刊论文
| 作者 | Wang, T; Yun, CH; Gu, SY; Chang, WR; Liang, DC |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-08-05 |
| 卷号 | 333期号:3页码:845-849 |
| 关键词 | Human nuclear cyclophilin Cyclophilin a Peptidyl-prolyl cis-trans isomerase Cyclosporin a |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.06.006 |
| 通讯作者 | Liang, dc() |
| 英文摘要 | Cyclophilins (cyps) are a widespreading protein family in living organisms and possess the activity of peptidyl-prolyl cis-trans isomerase (ppiase), which is inhibited by cyclosporin a (csa). the human nuclear cyclophilin (hcyp33) is the first protein which was found to contain two rna binding domains at the amino-terminus and a ppiase domain at the carboxyl-terminus. we isolated the hcyp33 gene from the human hematopoietic stem/progenitor cells and expressed it in escherichia coli, and determined the crystal structure of the c domain of hcyp33 at 1.88 angstrom resolution. the core structure is a beta-barrel covered by two alpha-helices. superposition of the structure of the c domain of hcyp33 with the structure of cypa suggests that the c domain contains ppiase active site which binds to csa. furthermore, c domain seems to be able to bind with the gag-encoded capsid (ca) of hiv-1 and may affect the viral replication of hiv-1. a key residue of the active site is changed from ala-103-cypa to ser-239-hcyp33, which may affect the ppiase domain/substrates interactions. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | HUMAN T-CELLS ; CYCLOPHILIN-A ; CYCLOSPORINE-A ; BINDING-PROTEIN ; INHIBITION ; COMPLEXES ; REPLICATION ; CALCINEURIN ; REFINEMENT ; RESOLUTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000230418800026 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378256 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liang, DC |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, T,Yun, CH,Gu, SY,et al. 1.88 angstrom crystal structure of the c domain of hcyp33: a novel domain of peptidyl-prolyl cis-trans isomerase[J]. Biochemical and biophysical research communications,2005,333(3):845-849. |
| APA | Wang, T,Yun, CH,Gu, SY,Chang, WR,&Liang, DC.(2005).1.88 angstrom crystal structure of the c domain of hcyp33: a novel domain of peptidyl-prolyl cis-trans isomerase.Biochemical and biophysical research communications,333(3),845-849. |
| MLA | Wang, T,et al."1.88 angstrom crystal structure of the c domain of hcyp33: a novel domain of peptidyl-prolyl cis-trans isomerase".Biochemical and biophysical research communications 333.3(2005):845-849. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。