RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model
文献类型：期刊论文
| 作者 | Hu, Chunxiu1,2; van Dommelen, Judith1; van der Heijden, Rob1; Spijksma, Gerwin1; Reijmers, Theo H.1; Wang, Mei3,4; Slee, Elizabeth5; Lu, Xin5; Xu, Guowang2; van der Greef, Jan1,3,4 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 7期号:11页码:4982-4991 |
| 关键词 | Lipidomics Lipid Profiling Metabolite Profiling Metabolomics FTMS p53 Mouse Model |
| 产权排序 | 2;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | a reversed-phase liquid chromatography-linear ion trap-fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. with the use of a fused-core c(8) column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single lc-ms run. the method was fully validated and the analytical characteristics such as linearity (r(2), 0.994-1.000), limit of detection (0.08-1.28 mu g/ml plasma), repeatability (rsd, 2.7-7.9%) and intermediate precision (rsd, 2.7-15.6%) were satisfactory. the method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | performance liquid-chromatography ; tandem mass-spectrometry ; bronchoalveolar lavage fluid ; biological samples ; membrane phospholipids ; quantitative-analysis ; crude extracts ; identification ; ms ; phosphatidylcholine |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000260792000038 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101153]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Leiden Univ, LACDR, Div Analyt Biosci, NL-2300 RA Leiden, Netherlands 2.Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.SU BioMed, NL-3700 AJ Zeist, Netherlands 4.TNO Qual Life, NL-3700 AJ Zeist, Netherlands 5.Univ Oxford, Ludwig Inst Canc Res, Oxford OX3 7DQ, England |
| 推荐引用方式 GB/T 7714 | Hu, Chunxiu,van Dommelen, Judith,van der Heijden, Rob,et al. RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model[J]. journal of proteome research,2008,7(11):4982-4991. |
| APA | Hu, Chunxiu.,van Dommelen, Judith.,van der Heijden, Rob.,Spijksma, Gerwin.,Reijmers, Theo H..,...&Hankemeier, Thomas.(2008).RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model.journal of proteome research,7(11),4982-4991. |
| MLA | Hu, Chunxiu,et al."RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model".journal of proteome research 7.11(2008):4982-4991. |
入库方式： OAI收割
来源：大连化学物理研究所
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