Improving phage antibody chip by pvii display system
文献类型：期刊论文
| 作者 | Liao, W; Hong, L; Wei, F; Zhu, SG; Zhao, XS |
| 刊名 | Acta physico-chimica sinica
 |
| 出版日期 | 2005-05-01 |
| 卷号 | 21期号:5页码:508-511 |
| 关键词 | Protein chips Phage-display antibody P iii display system Pviii display system Scfv specific to thrombin |
| ISSN号 | 1000-6818 |
| DOI | 10.3866/pku.whxb20050510 |
| 通讯作者 | Zhu, sg(zhusg@pku.edu.cn) |
| 英文摘要 | Phage antibody chips, prepared by coupling the recombinant phage displaying scfv with carboxyl-terminated silicon, can be applied in detecting proteins and proteomes. in previous work, we immobilized phages displaying scfv on the coat protein piii on silicon surface as model chips. to improve the sensitivity and the ratio of signal to noise, in this work, scfv specific binding to thrombin was displayed by pviii display system, which is the major coat protein on the phage. as the results of changing from piii to pviii display, the sensitivity and the ratio of signal to noise of chips were enhanced prominently, due to the increase of the copy number and the decrease of steric hindrance. it is concluded that scfv fused to the coat protein pviii is a promising candidate for the preparation of novel protein chips. |
| WOS关键词 | PROTEIN MICROARRAYS ; LIBRARIES ; EXPRESSION ; SURFACE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Physical |
| 语种 | 英语 |
| WOS记录号 | WOS:000229305800010 |
| 出版者 | PEKING UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378305 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, SG |
| 作者单位 | 1.Peking Univ, Dept Biol Chem, Coll Chem & Chem Engn, State Key Lab Mol Dynam & Stable Struct, Beijing 100871, Peoples R China 2.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liao, W,Hong, L,Wei, F,et al. Improving phage antibody chip by pvii display system[J]. Acta physico-chimica sinica,2005,21(5):508-511. |
| APA | Liao, W,Hong, L,Wei, F,Zhu, SG,&Zhao, XS.(2005).Improving phage antibody chip by pvii display system.Acta physico-chimica sinica,21(5),508-511. |
| MLA | Liao, W,et al."Improving phage antibody chip by pvii display system".Acta physico-chimica sinica 21.5(2005):508-511. |
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来源：中国科学院大学
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