Hydrogen peroxide sensor based on horseradish peroxidase immobilized on a silver nanoparticles/cysteamine/gold electrode
文献类型：期刊论文
| 作者 | Ren, CB; Song, YH; Li, Z; Zhu, GY |
| 刊名 | Analytical and bioanalytical chemistry
 |
| 出版日期 | 2005-03-01 |
| 卷号 | 381期号:6页码:1179-1185 |
| 关键词 | Biosensor Horseradish peroxidase Self-assemble Silver nanoparticles Afm |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-004-3032-0 |
| 通讯作者 | Zhu, gy() |
| 英文摘要 | A third-generation hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (hrp) on a gold electrode modified with silver nanoparticles. a freshly-cleaned gold electrode was first immersed in a cysteamine-ethanol solution, and then silver nanoparticles were immobilized on the cysteamine monolayer, and finally hrp was adsorbed onto the surfaces of the silver nanoparticles. this self-assemble process was examined via atomic force microscopy (afm). the immobilized horseradish peroxidase exhibited an excellent electrocatalytic response toward the reduction of hydrogen peroxide. the linear range of the biosensor was 3.3 mu m to 9.4 mm, and the detection limit was estimated to be 0.78 mu m. moreover, the biosensor exhibited a fast response, high sensitivity, good reproducibility, and long-term stability. |
| WOS关键词 | MODIFIED GOLD ELECTRODE ; DIRECT ELECTROCHEMISTRY ; COLLOIDAL GOLD ; CYTOCHROME-C ; NANOPARTICLES ; AU ; MONOLAYERS ; PARTICLES ; PROTEINS ; FILMS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000227946500010 |
| 出版者 | SPRINGER HEIDELBERG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378335 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, GY |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, CB,Song, YH,Li, Z,et al. Hydrogen peroxide sensor based on horseradish peroxidase immobilized on a silver nanoparticles/cysteamine/gold electrode[J]. Analytical and bioanalytical chemistry,2005,381(6):1179-1185. |
| APA | Ren, CB,Song, YH,Li, Z,&Zhu, GY.(2005).Hydrogen peroxide sensor based on horseradish peroxidase immobilized on a silver nanoparticles/cysteamine/gold electrode.Analytical and bioanalytical chemistry,381(6),1179-1185. |
| MLA | Ren, CB,et al."Hydrogen peroxide sensor based on horseradish peroxidase immobilized on a silver nanoparticles/cysteamine/gold electrode".Analytical and bioanalytical chemistry 381.6(2005):1179-1185. |
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来源：中国科学院大学
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