Neuroprotective effects of cyclooxygenase-2 inhibitor celecoxib against toxicity of lps-stimulated macrophages toward motor neurons
文献类型：期刊论文
| 作者 | Huang, Y; Liu, J; Wang, LZ; Zhang, WY; Zhu, XZ |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 26期号:8页码:952-958 |
| 关键词 | Amyotrophic lateral sclerosis Celecoxib Macrophage Nsc34 cell Lipopolysaccharide Cyclooxygenase-2 Prostaglandin e2 Nitric oxide Reactive oxygen species |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2005.00136.x |
| 通讯作者 | Zhu, xz() |
| 英文摘要 | Aim: to establish an in vitro injured motor neuronal model and investigate the neuroprotective effects and possible mechanism of celecoxib a selective, cyclooxygenase-2 (cox-2) inhibitor, on this model. methods: after macrophages were stimulated with lipopolysaccharide (lps)+interferon-gamma (ifn-gamma) in the presence or absence of celecoxib for 24 h, the cell-free supernatant of lps-stimulated macrophages was transferred to the culture of nsc34 cells. viability of nsc34 cells was assessed by mtt assay after a further 24 h and 72 h incubation. after macrophages were stimulated by lps+ifn-gamma for 12 h or 24 h, the release of prostaglandin e-2 (pge(2)), nitric oxide (no), reactive oxygen species (ros), tumor necrosis factor alpha (tnf-alpha) and interleukin-1 beta (il-1 beta) from macrophages was measured by radioimmunoassay, griess assay, fluorescence assay and enzyme-linked immunosorbent assay, respectively. the mrna levels of cox-2, inducible nitric oxide synthase (inos), tnf-alpha and il-1 beta in macrophages were determined by reverse transcription-polymerase chain reaction after macrophages were stimulated for 6 h and 12 h. results: the supernatant of lps-stimulated mouse macrophages induced the death of nsc34 cells and celecoxib protected the nsc34 cells against this toxicity. the lps-induced increases in the release of pge(2), no, tnf-alpha and il-1 beta from macrophages were attenuated by pre-treatment with celecoxib. however, celecoxib showed no effect on the ros levels upregulated by lps+ifn-gamma in the macrophage supernatant. the mrna levels of cox-2, inos, tnf-alpha and il-1 beta were increased in lps-activated macrophages and, except cox-2, reduced by pre-treatment with celecoxib. conclusion: an in vitro injured motor neuronal model was established by using the toxicity of lps-stimulated mouse macrophages toward motor neuronal nsc34 cells. in this model, celecoxib exerted neuroprotective effects on motor neurons via an inhibition of the neurotoxic secretions from activated macrophages. |
| WOS关键词 | AMYOTROPHIC-LATERAL-SCLEROSIS ; NITRIC-OXIDE SYNTHASE ; TRANSGENIC MOUSE MODEL ; PROSTAGLANDIN E-2 ; SPINAL-CORD ; BRAIN MACROPHAGES ; GENE-EXPRESSION ; MICROGLIA ; CELLS ; ALS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000231255700007 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378349 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Shanghai Inst Mat Med,Dept Pharmacol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Y,Liu, J,Wang, LZ,et al. Neuroprotective effects of cyclooxygenase-2 inhibitor celecoxib against toxicity of lps-stimulated macrophages toward motor neurons[J]. Acta pharmacologica sinica,2005,26(8):952-958. |
| APA | Huang, Y,Liu, J,Wang, LZ,Zhang, WY,&Zhu, XZ.(2005).Neuroprotective effects of cyclooxygenase-2 inhibitor celecoxib against toxicity of lps-stimulated macrophages toward motor neurons.Acta pharmacologica sinica,26(8),952-958. |
| MLA | Huang, Y,et al."Neuroprotective effects of cyclooxygenase-2 inhibitor celecoxib against toxicity of lps-stimulated macrophages toward motor neurons".Acta pharmacologica sinica 26.8(2005):952-958. |
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来源：中国科学院大学
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