Immobilization of dna on 11-mercaptoundecanoic acid-modified gold(111) surface for atomic force microscopy imaging
文献类型：期刊论文
| 作者 | Song, YH; Li, Z; Liu, ZG; Wei, G; Wang, L; Sun, LL |
| 刊名 | Microscopy research and technique
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 68期号:2页码:59-64 |
| 关键词 | Atomic force microscopy (afm) Dna Gold (111) Self-assembled monolayers (sams) 11-mercaptoundecanoic acid (muda) |
| ISSN号 | 1059-910X |
| DOI | 10.1002/jemt.20235 |
| 通讯作者 | Li, z() |
| 英文摘要 | Immobilized dna on preformed 11-mercaptoundecanoic acids (muda) self-assembled monolayers (sams) on a gold (111) surface was bound by a divalent cation bridges was imaged by atomic force microscopy (afm). the dna immobilization was attributed to the formation of ionic bridges between the carboxylate groups of muda and the phosphate groups of dna. afm images revealed that dna molecules could be immobilized strongly enough to permit stable and reproducible imaging. the effect of different bridge cations, such as mg2+, zn2+ and cu2+, and the ph of dna assembled solution on immobilization and conformation of dna was studied. plasmid dna pbr 322/pst i molecules were straightened by using a molecular combing technique on the muda surface. |
| WOS关键词 | SELF-ASSEMBLED MONOLAYERS ; SUPERCOILED DNA ; GOLD ELECTRODES ; MICA ; COMPLEXES ; TRANSITION ; HUMIDITY ; AU(111) ; BINDING ; WATER |
| WOS研究方向 | Anatomy & Morphology ; Life Sciences & Biomedicine - Other Topics ; Microscopy |
| WOS类目 | Anatomy & Morphology ; Biology ; Microscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000233026000001 |
| 出版者 | WILEY-LISS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378359 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Z |
| 作者单位 | Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Grad Sch, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, YH,Li, Z,Liu, ZG,et al. Immobilization of dna on 11-mercaptoundecanoic acid-modified gold(111) surface for atomic force microscopy imaging[J]. Microscopy research and technique,2005,68(2):59-64. |
| APA | Song, YH,Li, Z,Liu, ZG,Wei, G,Wang, L,&Sun, LL.(2005).Immobilization of dna on 11-mercaptoundecanoic acid-modified gold(111) surface for atomic force microscopy imaging.Microscopy research and technique,68(2),59-64. |
| MLA | Song, YH,et al."Immobilization of dna on 11-mercaptoundecanoic acid-modified gold(111) surface for atomic force microscopy imaging".Microscopy research and technique 68.2(2005):59-64. |
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来源：中国科学院大学
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