Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups
文献类型：期刊论文
| 作者 | Liang, Aiye1,2; Liu, Xiaojun1,2; Du, Yuguang1; Wang, Keyi3; Lin, Bingcheng1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 29期号:6页码:1286-1290 |
| 关键词 | capillary zone electrophoresis granulocyte colony-stimulating factor heparin interaction sulfate group |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | heparin mediates fundamental biological mechanisms through interaction with proteins. previously, we have shown that standard heparin binds to granulocyte colony-stimulating factor (g-csf) with an affinity of 4.8 x 10(5) m-1. to further study the structural features in heparin that are responsible for this interaction, we studied the bindings of g-csf and n-desulfated and 2,3-o-desulfated heparin by cze. results showed that the n-desulfated heparin had a similar affinity for g-csf ((5.4 +/- 0.9) x 10(5) m-1), but the 2,3-o-desulfated heparin had a 1000-fold lower affinity ((3.4 +/- 1.2) x 10(2) m-1) in comparison to standard heparin. the results showed that 2,3-o-sulfate groups are more important than n-sulfate groups in heparin-g-csf interaction. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | fibroblast-growth-factor ; performance frontal analysis ; herpes-simplex-virus ; affinity capillary-electrophoresis ; in-vivo ; receptor ; complex ; protein ; identification ; proteoglycan |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000254882800010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101183]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Aiye,Liu, Xiaojun,Du, Yuguang,et al. Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups[J]. electrophoresis,2008,29(6):1286-1290. |
| APA | Liang, Aiye,Liu, Xiaojun,Du, Yuguang,Wang, Keyi,&Lin, Bingcheng.(2008).Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups.electrophoresis,29(6),1286-1290. |
| MLA | Liang, Aiye,et al."Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups".electrophoresis 29.6(2008):1286-1290. |
入库方式： OAI收割
来源：大连化学物理研究所
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