Hypoxia-mimetic agents desferrioxamine and cobalt chloride induce leukemic cell apoptosis through different hypoxia-inducible factor-1 alpha independent mechanisms
文献类型：期刊论文
| 作者 | Guo, M; Song, LP; Jiang, Y; Liu, W; Chen, GQ |
| 刊名 | Apoptosis
 |
| 出版日期 | 2006 |
| 卷号 | 11期号:1页码:67-77 |
| 关键词 | Apoptosis Cobalt chloride Desferrioxamine Hif-1 alpha Leukemia |
| ISSN号 | 1360-8185 |
| DOI | 10.1007/s10495-005-3085-3 |
| 通讯作者 | Chen, gq(chengq@shsmu.edu.cn) |
| 英文摘要 | Hypoxia presents pro-apoptotic and anti-apoptotic biphasic effects that appear to be dependent upon cell types and conditions around cells. the substantial reports demonstrated that commonly used hypoxia-mimetic agents cobalt chloride (cocl(2)) and desferrioxamine (dfo) could also induce apoptosis in many different kinds of cells, but the mechanism was poorly understood. in this work, we compare the apoptosis-inducing effects of these two hypoxia-mimetic agents with acute myeloid leukemic cell lines nb4 and u937 as in vitro models. the results show that both of them induce these leukemic cells to undergo apoptosis with a loss of mitochondrial transmembrane potentials (delta psi m), the activation of caspase-3/8 and the cleavage of anti-apoptotic protein mcl-1, together with the accumulation of hypoxia-inducible factor-1 alpha (hif-1 alpha) protein, a critical regulator for the cellular response to hypoxia. metavanadate and sodium nitroprusside significantly abrogate dfo rather than cocl(2)-induced mitochondrial delta psi m collapse, caspase-3/8 activation, mcl-1 cleavage and apoptosis, but they fail to influence dfo and cocl(2)-induced hif-1 alpha protein accumulation. moreover, inducible expression of hif-1 alpha gene dose not alter dfo and cocl(2)-induced apoptosis in u937 cells. in conclusion, these results propose that although both dfo and cocl(2)-induced leukemic cell apoptosis by mitochondrial pathway-dependent and hif-1 alpha-independent mechanisms, dfo and cocl(2)-induced apoptosis involves different initiating signal pathways that remain to be investigated. |
| WOS关键词 | NITRIC-OXIDE ; IRON CHELATORS ; MYELOID-LEUKEMIA ; ARSENIC TRIOXIDE ; FACTOR 1-ALPHA ; PROTEIN ; CANCER ; FACTOR-1-ALPHA ; ACTIVATION ; GROWTH |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000234858200007 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378461 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, GQ |
| 作者单位 | 1.Shanghai Inst Biol Sci, Hlth Sci Ctr, Shanghai 200025, Peoples R China 2.Chinese Acad Sci, Shanghai Jiao Tong Univ, Sch Med, Grad Sch, Shanghai 200025, Peoples R China 3.Shanghai Med Univ 2, Shanghai, Peoples R China 4.SJU SM, Key Lab Cell Differentiat & Apoptosis, Dept Pathophysiol, Shanghai 200025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, M,Song, LP,Jiang, Y,et al. Hypoxia-mimetic agents desferrioxamine and cobalt chloride induce leukemic cell apoptosis through different hypoxia-inducible factor-1 alpha independent mechanisms[J]. Apoptosis,2006,11(1):67-77. |
| APA | Guo, M,Song, LP,Jiang, Y,Liu, W,&Chen, GQ.(2006).Hypoxia-mimetic agents desferrioxamine and cobalt chloride induce leukemic cell apoptosis through different hypoxia-inducible factor-1 alpha independent mechanisms.Apoptosis,11(1),67-77. |
| MLA | Guo, M,et al."Hypoxia-mimetic agents desferrioxamine and cobalt chloride induce leukemic cell apoptosis through different hypoxia-inducible factor-1 alpha independent mechanisms".Apoptosis 11.1(2006):67-77. |
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来源：中国科学院大学
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