Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS
文献类型：期刊论文
| 作者 | Zhou, Xiaomian; Shen, Zheng; Li, Dazhi; He, Xinya; Lin, Bingcheng |
| 刊名 | talanta
 |
| 出版日期 | 2007-04-30 |
| 卷号 | 72期号:2页码:561-567 |
| 关键词 | microchip non-gel sieving electrophoresis actinomycin D DNA interaction electrospray ionization mass spectrometry |
| 产权排序 | 2;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | fwn the present study, the interactions between actinomycin d (actd) and single stranded dna (ssdna) 5'-cgtaaccaactgcaacgt-3 and a duplex stranded dna (dsdna) with this sequence were investigated by microchip-based non-gel sieving electrophoresis and electrospray ionization mass spectrometry (esi-ms). the ssdna was designed according to the conserved regions of open reading frame lb (replicase 113) following the tor 2 sars genome sequence of 15611-15593. the binding constants of the interactions between actd and ssdna/dsdna were (8.3 +/- 0.32) x 10(6) m-1 (ssdna) and (2.8 +/- 0.02) x 10(5)m(-1) (dsdna), respectively, calculated from microchip electrophoresis via scatchard plot. the binding stoichiometries were 1: 1 (single/1actd molecule) and 1:2 (duplex/2actd molecules) calculated from microchip electrophoresis, and the results were further verified by esi-ms. the results obtained by these two methods indicated that actd) bound much more tightly to ssdna used in this work than dsdna. furthermore, this is shown that the microchip-based non-gel sieving electrophoresis method is a rapid, highly sensitive and convenient method for the studies of interactions between dna and small molecule drugs. (c) 2006 published by elsevier b.v. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | affinity capillary-electrophoresis ; single-stranded-dna ; mass-spectrometry ; microfluidic chip ; fluorescence detection ; noncovalent complexes ; zone-electrophoresis ; drug-interaction ; binding drugs ; oligodeoxynucleotides |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000246321200034 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101195]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Guangzhou First Municipal Peoples Hosp, Affiliated Guangzhou Med Coll, Dept Lab Med, Guangzhou 510180, Peoples R China 2.Chinese Acad Sci, Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Xiaomian,Shen, Zheng,Li, Dazhi,et al. Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS[J]. talanta,2007,72(2):561-567. |
| APA | Zhou, Xiaomian,Shen, Zheng,Li, Dazhi,He, Xinya,&Lin, Bingcheng.(2007).Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS.talanta,72(2),561-567. |
| MLA | Zhou, Xiaomian,et al."Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS".talanta 72.2(2007):561-567. |
入库方式： OAI收割
来源：大连化学物理研究所
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