Simultaneous determination of baicalin, rhein and berberine in rat plasma by column-switching high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Yi, L; Gao, JP; Xu, X; Dai, LX |
| 刊名 | Journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2006-06-21 |
| 卷号 | 838期号:1页码:50-55 |
| 关键词 | Hplc Plasma Column-switching Traditional chinese medicine Baicalin Rhein Berberine |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2006.04.015 |
| 通讯作者 | Xu, x() |
| 英文摘要 | A simple hplc method using column-switching and ultraviolet detection was developed for the simultaneous determination of baicalin (ba), rhein (rh) and berberine (be) in rat plasma. plasma samples were injected directly onto a capcell pak mf c-8 column (150 mm x 4.6 mm i.d.) to remove protein and to be pre-separated by an isocratic elution using 50 mmol/l phosphate sodium (ph 6.85)-acetonitrile (10:1, v/v). after the drug-containing fractions were transferred to a kromasil c column (150 nun x 4.6 turn i.d.) by a valve switching step, the valve position was switched back and the main separation was performed by an isocratic elution using triethylamine adjusted 20 mmol/l phosphoric acid (ph 6.78)-acetonitrile (4: 1, v/v). the flow rate was always 1.0 ml/min. the calibration curve showed excellent linear relationship (r >= 0.9997) over the concentration range of 0.4-7.9 mu g/ml for baicalin, 0.2-7.8 mu g/ml for rhein and 0.4-7.7 mu g/ml for berberine in rat plasma. the intra- and inter-day assay precisions (r.s.d.) of three analytes were in the range of 0.34-4.3% and the accuracies were between 98.0% and 102.4%. their recoveries were all greater than 95%. the method was successfully applied to the multi-constituents plasma concentration-time curve study after oral administration of a traditional chinese medicine prescription xiexin-tang in rats. (c) 2006 elsevier b.v. all rights reserved. |
| WOS关键词 | HUANGQIN-TANG ; CONSTITUENTS ; ASSAY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000238602100010 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378471 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, X |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, Lab Analyt Chem, State Key Lab Organomet Chem, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yi, L,Gao, JP,Xu, X,et al. Simultaneous determination of baicalin, rhein and berberine in rat plasma by column-switching high-performance liquid chromatography[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2006,838(1):50-55. |
| APA | Yi, L,Gao, JP,Xu, X,&Dai, LX.(2006).Simultaneous determination of baicalin, rhein and berberine in rat plasma by column-switching high-performance liquid chromatography.Journal of chromatography b-analytical technologies in the biomedical and life sciences,838(1),50-55. |
| MLA | Yi, L,et al."Simultaneous determination of baicalin, rhein and berberine in rat plasma by column-switching high-performance liquid chromatography".Journal of chromatography b-analytical technologies in the biomedical and life sciences 838.1(2006):50-55. |
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来源：中国科学院大学
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