Sequence analysis of mrna polyadenylation signals of rice genes
文献类型：期刊论文
| 作者 | Lu Ying; Gao Chenxi; Han Bin |
| 刊名 | Chinese science bulletin
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 51期号:9页码:1069-1077 |
| 关键词 | Polyadenylation signals U-rich downstream elements Cdna Expressed sequences tags Rna processing Rice |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-006-1069-5 |
| 通讯作者 | Han bin(bhan@ncgr.ac.cn) |
| 英文摘要 | The formation of eukaryotic mrnas involves the cleavage and polyadenylation of pre-mrnas. to investigate the sequence requirement of putative polyadenylation signals (pass), poly(a) sites and downstream elements (dues) in t-end-processing in rice, we compared expressed sequences tags (ests) with poly(a) extremity to full-length cdna sequences and constructed a database of 12969 pre-mrna sequences in -40-+40 nt surrounding the poly(a) sites, which were from 9953 genes. the alternative poly(a) sites were revealed in approximately 25% of mrnas. nearly 80% of pre-mrnas showed stringent requirement of the ya (ca or ua) at poly (a) sites for polyadenylation. about 7.9% had the aauaaa signals on -40--1 nt upstream of the poly(a) sites. over 60% of mrnas probably used the one- or two-base variants of aauaaa hexamers as their pass in 3' fragments. the single-base variants of aaugaa revealed the high frequency in 11.5% of 3' fragments. the dues were detected in 90% of pre-mrnas, especially more than half of the pre-mrnas with multi-base variants of aauaaa had the dues surrounding the poly(a) site. the location of due is also important for defining the cleavage site. although most of the rice pre-mrnas did not contain aauaaa signal, the existence of downstream elements ensured the efficiency of cleavage-polyadenylation. |
| WOS关键词 | DOWNSTREAM ELEMENTS ; REPRESENTED WORDS ; SITE SELECTION ; CLEAVAGE ; SECONDARY ; AAUAAA ; PLANTS ; USAGE ; END |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000238882500009 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378487 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Han Bin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Ctr Gene Res, Shanghai 200223, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu Ying,Gao Chenxi,Han Bin. Sequence analysis of mrna polyadenylation signals of rice genes[J]. Chinese science bulletin,2006,51(9):1069-1077. |
| APA | Lu Ying,Gao Chenxi,&Han Bin.(2006).Sequence analysis of mrna polyadenylation signals of rice genes.Chinese science bulletin,51(9),1069-1077. |
| MLA | Lu Ying,et al."Sequence analysis of mrna polyadenylation signals of rice genes".Chinese science bulletin 51.9(2006):1069-1077. |
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来源：中国科学院大学
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