Neoplastic transformation of human diploid fibroblasts after long-term serum starvation
文献类型：期刊论文
| 作者 | Zhang, Jingyu; Wang, Xia; Zhao, Yanhong; Chen, Bing; Suo, Guangli; Dai, Jianwu |
| 刊名 | Cancer letters
 |
| 出版日期 | 2006-11-08 |
| 卷号 | 243期号:1页码:101-108 |
| 关键词 | Tumorigenesis Serum starvation Fibroblasts |
| ISSN号 | 0304-3835 |
| DOI | 10.1016/j.canlet.2005.11.022 |
| 通讯作者 | Dai, jianwu(jwdai@genetics.ac.cn) |
| 英文摘要 | Serum starvation for several days has been considered as a positive effect on the efficiency of nuclear transfer using donor cells. the effects of longer period serum starvation are not clear while similar starvation might occur in vitro maintained cells (i.e. tissue engineering products) and in vivo such as ischemia of human tissues or organs. we found human dermis fibroblasts were transformed for about 70 days caused by serum starvation (0.5% serum). the transformed cells became round and had more than one nucleolus. in 0.5% serum medium they kept almost constant growth rate as the normal fibroblasts in 10% serum medium. abnormal karyotype including aneuploidy and structural aberrations was observed. the transformed cells had high telomerase activities, in contrast, normal fibroblasts had no detectable telomerase activities. c-myc was up-regulated while cdk2, cyclin a, p21 were down in transformed cells. cell transplantation into scid nude mice confirmed that the cells had the capacity of forming solid tumors. the results indicated that long-term serum starvation could lead to cell chromosomal instability and transformation. (c) 2005 elsevier ireland ltd. all rights reserved. |
| WOS关键词 | HUMAN LUNG FIBROBLASTS ; NUCLEAR TRANSFER ; CELL-TRANSFORMATION ; MAMMALIAN-CELLS ; TELOMERASE ; FETAL ; EXPRESSION ; ELEMENTS ; GROWTH ; CANCER |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000242059200014 |
| 出版者 | ELSEVIER IRELAND LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378492 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dai, Jianwu |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jingyu,Wang, Xia,Zhao, Yanhong,et al. Neoplastic transformation of human diploid fibroblasts after long-term serum starvation[J]. Cancer letters,2006,243(1):101-108. |
| APA | Zhang, Jingyu,Wang, Xia,Zhao, Yanhong,Chen, Bing,Suo, Guangli,&Dai, Jianwu.(2006).Neoplastic transformation of human diploid fibroblasts after long-term serum starvation.Cancer letters,243(1),101-108. |
| MLA | Zhang, Jingyu,et al."Neoplastic transformation of human diploid fibroblasts after long-term serum starvation".Cancer letters 243.1(2006):101-108. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。