A periplasmic glutamate/aspartate binding protein from shigella flexneri: gene cloning, over-expression, purification and preliminary crystallographic studies of the recombinant protein
文献类型：期刊论文
| 作者 | Fan, CP; Zhu, DY; Lu, HX; Jin, Q; Wang, DC |
| 刊名 | Protein and peptide letters
 |
| 出版日期 | 2006 |
| 卷号 | 13期号:5页码:513-516 |
| 关键词 | Periplasmic glutamate/aspartate binding protein Shigella flexneri Crystallization Crystal data |
| ISSN号 | 0929-8665 |
| 通讯作者 | Wang, dc() |
| 英文摘要 | Periplasmic substrate binding proteins (psbps) are essential components of the bacterial periplasmic transport system, which transports a wide variety of nutrients from the periplasmic space to the cytoplasm. the glutamate/aspartate binding protein sfglu/aspbp is a unique psbp consisting of 279 amino acid residues. the sfglu/aspbp gene was cloned, over-expressed, and purified by immobilized metal ion affinity chromatography and size-exclusion chromatography, the recombinant protein sfglu/aspbp has been crystallized by the hanging-drop vapor-diffusion method and its xray diffraction data were collected at an atomic resolution of 1.15 angstrom. the crystals belong to the space group p2(1) with unit cell parameters: a=48.41 angstrom, b=68.18 angstrom, c=80.21 angstrom and beta = 98.78 degrees. there are two molecules per asymmetric unit. |
| WOS关键词 | ESCHERICHIA-COLI ; TRANSPORT-SYSTEM ; RESOLUTION ; PERMEASES ; SEQUENCE ; LEUCINE ; COMPLEX |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000237935900017 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378529 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, DC |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Ctr Struct & Mol Biol, Beijing 100101, Peoples R China 2.State Key Lab Mol Virol & Genet Engn, Beijing 100052, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, CP,Zhu, DY,Lu, HX,et al. A periplasmic glutamate/aspartate binding protein from shigella flexneri: gene cloning, over-expression, purification and preliminary crystallographic studies of the recombinant protein[J]. Protein and peptide letters,2006,13(5):513-516. |
| APA | Fan, CP,Zhu, DY,Lu, HX,Jin, Q,&Wang, DC.(2006).A periplasmic glutamate/aspartate binding protein from shigella flexneri: gene cloning, over-expression, purification and preliminary crystallographic studies of the recombinant protein.Protein and peptide letters,13(5),513-516. |
| MLA | Fan, CP,et al."A periplasmic glutamate/aspartate binding protein from shigella flexneri: gene cloning, over-expression, purification and preliminary crystallographic studies of the recombinant protein".Protein and peptide letters 13.5(2006):513-516. |
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来源：中国科学院大学
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