Mechanically driven proton conduction in single delta-free f0f1-atpase
文献类型：期刊论文
| 作者 | Liu Xiaolong; Zhang Xiaoai; Cui Yuanbo; Yue Jiachang; Luo Zhiyong; Jiang Peidong |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 347期号:3页码:752-757 |
| 关键词 | F0f1-atpase Mechanically driven proton flux Chromatophore |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.06.160 |
| 通讯作者 | Jiang peidong(yuejc@sun5.ibp.ac.cn) |
| 英文摘要 | In order to observe mechanically driven proton flux in f0f1-atpase coupled with artificial driven rotation on f, simultaneously, a double channel observation system was established. an artificial delta-free f0f1-atpase was constructed with alpha(3), beta(3), epsilon, gamma, and c(n) subunits as rotator and a, b(2) as stator. the chromatophore was immobilized on the glass surface through biotin-streptavidin-biotin system, and the magnetic bead was attached to the beta subunit of delta-free f0f1-atpase. the mechanically driven proton flux was indicated by the fluorescence intensity change of fluorescein reference standard (f1300) and recorded by a cooled digital ccd camera. the mechanochemical coupling stoichiometry between f-0 and f-1 is about 4.15 +/- 0.2h(+)/rev when the magnetic field rotated at 0.33 hz (rps). (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | ATP SYNTHASE ; COUPLING FACTOR ; MEMBRANE ; COMPLEX |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000239587200029 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378540 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang Peidong |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Xian Jiaotong Univ, Sch Life Sci & Technol, Key Lab Biomed Informat Engn Educ, Xian 710049, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Xiaolong,Zhang Xiaoai,Cui Yuanbo,et al. Mechanically driven proton conduction in single delta-free f0f1-atpase[J]. Biochemical and biophysical research communications,2006,347(3):752-757. |
| APA | Liu Xiaolong,Zhang Xiaoai,Cui Yuanbo,Yue Jiachang,Luo Zhiyong,&Jiang Peidong.(2006).Mechanically driven proton conduction in single delta-free f0f1-atpase.Biochemical and biophysical research communications,347(3),752-757. |
| MLA | Liu Xiaolong,et al."Mechanically driven proton conduction in single delta-free f0f1-atpase".Biochemical and biophysical research communications 347.3(2006):752-757. |
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来源：中国科学院大学
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